胶质瘤中NDRG2,PKC-α mRNA表达及相关性研究  

Expressions of NDRG2 and PKC-α mRNA in human glioma and their correlation

作　　者：周彬[1] 邓艳春[1] 刘新平[2] 刘南[1] 唐琢[1] 张健[2] 程浩然[1] 

机构地区：[1]第四军医大学西京医院神经内科 [2]第四军医大学基础部生物化学与分子生物学教研室,陕西西安710033

出　　处：《第四军医大学学报》2008年第9期782-784,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30672168)

摘　　要：目的:研究胶质瘤中蛋白激酶PKC-α mRNA表达与NDRG2 mRNA表达的相关性,寻找可能与NDRG2相互作用的上下游分子.方法:用实时定量PCR技术检测26例脑胶质瘤及相应瘤旁组织中NDRG2和PKC-α mRNA表达量.结果:26例脑胶质瘤NDRG2 mRNA表达低于瘤旁组织(0.95±0.16vs1.54±0.18,P<0.05),PKC-α mRNA表达高于瘤旁组织(1.23±0.11vs0.97±0.18,P<0.05),且随胶质瘤恶性程度增高,NDRG2表达逐渐减少(P<0.05),PKC-α表达有逐渐增加的趋势.NDRG2与PKC-α表达无相关(P>0.05).结论:NDRG2,PKC-α可能与胶质瘤的发生发展及恶性演变有关,在mRNA表达水平,还不能认为NDRG2与PKC-α之间有相关性.AIM： To investigate the correlation between PKC-α and NDRG2 expressions in human glioma, so as to find the upstream or downstream molecular interacting with NDRG2. METHODS： Real time PCR was used to analyze the expressions of PKC-α and NDRG2 mRNA in 26 pairs of glioma and adjacent normal tissues. RESULTS： Compared with adjacent normal tissues, the expression level of NDRG2 mRNA in glioma tissues was reduced significantly [（0. 95±0. 16） vs （1. 54±0. 18）, P 〈 0.05, but PKC-α was increased significantly （ 1.23±0.11 ） vs （0.97±0. 18 ）, P 〈 0.05 ]. And with pathologic grade increasing, there was a trend for NDRG2 level to reduce （ P 〈 0.05） , however PKC-α level to increase （ P 〉 0.05）. But there was no statistical correlation between PKC-α and NDRG2. CONCLUSION ： The aberrant expressions of NDRG2 and PKC-α are possibly associated with the malignant transformation of glioma, but at mRNA level, PKC-α possibly has no correlation with NDRG2 in glioma.

关 键 词：神经胶质瘤 NDRG2基因 蛋白激酶C-Α 实时定量PCR 

分 类 号：R74[医药卫生—神经病学与精神病学]