载两性霉素B的聚乳酸纳米粒对大鼠血液系统(溶血)及肝肾功能的影响  

Primary study on the effects of AmB-PLA nanoparticles on liver,kidney and hematological system of rats

作　　者：徐楠[1] 郁晓[2] 温海[1] 陈江汉[1] 

机构地区：[1]第二军医大学长征医院皮肤科,上海200003 [2]上海同济大学科学与工程学院,上海200092

出　　处：《中国真菌学杂志》2008年第2期65-68,共4页Chinese Journal of Mycology

摘　　要：目的研究携载两性霉素B的聚乳酸纳米粒(AmB-PLA-NP)对大鼠肝、肾及血液系统的影响。方法将大鼠随机分4组,分别经尾静脉注射AmB、AmB-PLA-NP、PLA-NP(聚乳酸纳米粒)及表面活性剂聚山梨酯-80,定时取血检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、尿素氮(BUN)、肌酐(Cr)及红细胞(RBC)、血红蛋白(Hb)、白细胞(WBC)、血小板(PLT)等指标。结果AmB组大鼠给药前及给药后1d、1周的RBC分别为(5.84±0.37)×1012/L、(4.302±0.3)×1012/L和(3.3±0.37)×1012/L,AmB-PLA-NP组分别为(6.142±0.55)×1012/L、(6.38±0.35)×1012/L和(6.14±0.18)×1012/L,溶血反应明显降低;AmB组给药后ALT及AST水平显著升高,分别为(1059.2±119.22)μmol/L、(466.6±357.30)μmol/L(给药1d后)和(1755±175.39)μmol/L、(2684.2±494.74)μmol/L(给药1周后),而AmB-PLA-NP组、PLA组及聚山梨酯-80组的大鼠肝、肾功能未发生明显变化。结论AmB-PLA-NP能够显著降低AmB对肝、肾及血液系统的毒副作用。Objective To evaluate the side-effects of the prepared AmB-PLA nanoparticles on liver, kidney and hematological system of rats. Methods Experimental animals were divided into 4 groups. AmB, AmB-PLA-NP coated with polysorbate-80, PLA-NP and polysorbate-80 were injected into rats via tail vein. Items such as ALT, AST, BUN, Cr, RBC, Hb, WBC and PLT were tested before injection or 1 dorl w later. Results RBC level in AraB group raised from （5.84±0.37）×10^12/L to （4.302±0.3） ×10^12/L and（3.3 ±0.37） ×10^12/L on day 1 and day 7 after injection, respectively. ALT, AST showed a continuous rise which went to （ 1059.2 ± 119.22） , （2466.6 ±357.30） μmol/L（ 1 d） and （ 1755 ± 175.39） , （2684.2 ±494.74） μmol/L（ 1 w） respectively. BUN level also showed a rising tendency to some extent. In AmB-PLA-NP group, RBC level was （ 6.38 v0.35 ） and （ 6.14 ±0.18 ） ×10^12/L on day 1, day 7 after treatment, which meant hemolytic reaction was mitigated. There was nearly no change in BUN, Cr or ALT, AST level in AmB-PLA-NP, AmB-NP or polysorbate 80 groups. Conclusion AmB-PLA-NP could evidently alleviate the damage of AmB to liver, kidney and hematological system.

关 键 词：两性霉素B 聚乳酸 纳米粒 副作用 

分 类 号：R453.2[医药卫生—治疗学]