1,3-环己二酮-甲醛作为荧光衍生试剂荧光分光光度法测定氨苄西林钠  

Fluorimetric determination of ampicillin sodium in pharmaceutical products using 1,3-cyclohexanediono and formaldehyde as fluorigenic reagents

作　　者：赵晓辉 马丽英[2] 姜吉刚[2] 王怀友[3] 

机构地区：[1]山东省商贸学校,济南250014 [2]滨州医学院化学系,滨州256603 [3]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《分析试验室》2008年第5期42-45,共4页Chinese Journal of Analysis Laboratory

基　　金：山东省自然科学基金(Y2006B31)项目资助

摘　　要：建立了用1,3-环己二酮-甲醛作为荧光衍生试剂测定氨苄西林钠的方法。氨苄西林钠与1,3-环己二酮-甲醛反应生成一种强荧光物质。其最大激发波长是336 nm,最大发射波长是420 nm。氨苄西林钠浓度在0.01～0.6μg/mL范围内与相对荧光强度呈线性关系,线性方程为ρ=0.0007931F-0.06133,线性相关系数为0.9998,相对标准偏差为1.8%,检出限为10 ng/mL,回收率为97.0%～105.9%。考察了pH、温度、放置时间、干扰离子等对测定的影响。此法可用于注射液中氨苄西林钠的测定。Fluorimetric determination of ampicillin sodium is described. The ampicillin sodium reacts with 1,3-cyclo- hexanediono and formaldehyde to form a compound which is a strong fluorescent substance. The measurement was carried out at 420 um with excitation at 336 urn. The linear relationship was obtained between the relative fluorescence intensity and the concentration of ampicillin sodium in the range of 0.01 - 0.6 μg/mL. The linear regression equation of the calibration graph is C = 0.0007931F - 0. 06133 （ C is concentration of ampicillin sodium （μg/mL） and F is relative fluorescence intensity in the equation）, with a correlation coefficient of 0. 9998 and the relative standard deviation of 1.8%. The detection limit is 10 ng/mL, and the recoveries are from 97.0 to 105.9%. This method can be used for the determination of ampicillin sodium in injection samples.

关 键 词：荧光分光光度法 氨苄西林钠 环己二酮 甲醛 

分 类 号：O657.3[理学—分析化学]