猪圆环病毒2型山东株全基因组的克隆与序列分析  

Cloning and sequencing of complete genome of porcine circovirus type 2 (PCV-2)Shandong strains

作　　者：刘方娜[1] 刁有祥[1] 高晓伟[2] 孟凡磊[1] 王妮[1] 王辉[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2008年第5期501-505,共5页Chinese Journal of Veterinary Science

基　　金：山东省重大科技攻关资助项目(011020102)

摘　　要：根据GenBank已发表的猪圆环病毒2型（PCV-2）全基因组序列,设计2对特异性引物,对山东省不同地区规模化猪场采集的疑似断奶仔猪多系统消耗综合征（PMWS）组织病料进行了PCV-2全基因组克隆和序列分析。结果表明,PCV-2核苷酸序列较稳定,不同地区8个PCV-2毒株全基因组序列均由1 767 bp组成,彼此间核苷酸序列同源性达97.3%～99.8%,亲缘关系密切;与GenBank已发表的PCV-2参考毒株的同源性介于95.6%～99.8%。而ORF2的核苷酸序列同源性只有91.6%～99.9%。According to the genomic sequence of PCV-2 published in GenBank, two pairs of primers were designed to amplify the complete genomes of PCV-2 from the tissue of the suspicious PMWS sampled in different districts of Shandong province. The results showed that the complete genome of every strain consisted of 1 767 nucleotides. Nucleotide sequence homology between the eight PCV-2 isolates were found to be 97.3%-99.8% respectively. The homologies were 95. 6%-99.8% as compared with the sequences of PCV-2 published in GenBank. But nucleotide sequence homology of ORF2 is 91.6%-99.9%.

关 键 词：猪圆环病毒 全基因组 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]