人参皂苷Rg1-菌体CpG对IBDV VP2基因重组真核表达质粒免疫效果的影响  

Contribution of gensinoside-Rg_1-CpG to the immune effect induced by eukaryotic expression recombinant plasmids containing VP2 gene vaccines against IBDV

作　　者：许小琴 李迎晓[2] 刘红梅[3] 韦旭斌[4] 秦爱建 

机构地区：[1]扬州大学,江苏扬州225009 [2]信阳农业高等专科学校,河南信阳464000 [3]安徽农业大学,安徽合肥230036 [4]吉林大学,吉林长春130062

出　　处：《中国兽医学报》2008年第5期523-526,共4页Chinese Journal of Veterinary Science

基　　金：国家高技术发展计划资助项目(2002AA245081);国家自然基金资助项目(30471272)

摘　　要：将构建的含鸡传染性法氏囊病病毒(IBDV)JS超强毒株VP2基因的重组真核表达质粒与人参皂苷Rg1和CpG DNA联合佐剂免疫SPF鸡,检测免疫后的抗体水平,并观察超强毒株攻毒后的免疫保护效果。试验结果显示:Rg1-CpG佐剂与重组真核表达质粒DNA疫苗联合免疫后的抗体水平比单一使用DNA疫苗高,二者间有显著性差异;Rg1-CpG佐剂组的发病率、死亡率均低于非佐剂组,保护率则高于非佐剂组,且有显著性差异;而与法氏囊病中等毒力(NF8株)活疫苗免疫组的抗体水平及保护率相一致。表明Rg1-CpG佐剂显著增强了IBDV DNA疫苗的免疫效果。The immune effect of gensinoside-Rg1-CpG on SPF chickens immunized by eukaryotic expression recombinant plasmids containing IBDV VP2 gene was investigated in this study, the specific antibody was detected and immune effects were observed after inoculation. Results showed that the antibody titre in SPF chickens immunized with eukaryotic expression recombinant plasmids containing IBDV VP2 gene vaccine and gensinoside-Rg1-CpG as adjuvant were significantly higher than those of chickens only immunized with DNA vaccine, both morbidity and mortality of chickens with use of gensinoside-Rg1-CpG were significantly lower than those of chickens without use of gensinoside-Rg1-CpG,the antibody titre and protective effect were equal to those of chickens immunized with IBDV NF8 cell line. The above data indicated that the immune effect of DNA vaccine against IBDVA was enhanced by gensinoside-Rg1-CpG as adjuvants.

关 键 词：IBDV DNA疫苗 人参皂苷RG1 CPG DNA 免疫效果 

分 类 号：S859[农业科学—临床兽医学]