猪H-FABP基因遗传多态性及与肌内脂肪含量的相关分析  

Genetic polymorphism of H-FABP gene and correlation analysis with IMF content in porcine

作　　者：高妍[1] 张永宏[2] 姜宁[1] 袁宝[1] 肖书奇[1] 赵中利[1] 张树敏[3] 赵志辉[2] 张嘉保[1] 

机构地区：[1]吉林大学实验动物中心,吉林长春130062 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]吉林省农业科学院畜牧分院,吉林公主岭136100

出　　处：《中国兽医学报》2008年第5期599-603,共5页Chinese Journal of Veterinary Science

基　　金：吉林省重大科技专项资助(20065019)

摘　　要：采用PCR-RFLP的方法分析了松辽黑猪、军牧1号白猪、PIC猪、长白猪、大白猪、杜洛克猪和松野杂交猪共计127头猪H-FABP基因的遗传多态性。结果表明:(1)在5′-上游区的HinfⅠ多态位点上,所有试验猪种中均发现了多态性,在第二内含子的HaeⅢ多态位点上,所有被测猪种也都存在变异,在第二内含子的MspI多态位点上,松辽黑猪、长白猪和松野杂交猪仅表现为AA型,其他猪种均表现出多态性;(2)7个猪群的基因频率和基因型频率都达到了Hardy-Weinberg平衡状态(P>0.05);(3)松辽黑猪遗传变异对肌内脂肪含量的影响:HH基因型极显著高于Hh基因型(P<0.01),而Hh基因型又极显著高于hh基因型(P<0.01),最小二乘均值为:2.760>2.598>2.335,dd和Dd基因型分别极显著高于DD基因型(P<0.01),最小二乘均值为:2.763>2.732>2.347,结果提示:可通过提高"HH-dd"基因型的频率来增加松辽黑猪肌内脂肪含量,从而改善松辽黑猪的肉质。The genetic polymorphism of H-FABP gene in 127 pigs including Songliao black pig, Junmuyihao white pig, PIC pig, Landrace pig, Large White pig, Duroc pig and Songliao-Boar crossing pig was detected by PCR - RFLP. The results were as follows： （1） All the detected pig breeds varied at the Hinf I polymorphism locus of 5＇- upstream region,All the tested pig breeds varied at the Hae Ⅲ polymorphism locus of the second intron region, Songliao black pig ,Landrace pig and Songliao-Boar crossing pig only revealed AA genotype while other pig breeds revealed polymorphism at the MspI polymorphism locus of the second intron region; （2） all gene frequency and genotype frequency of seven porcine populations achieved Hardy-Weinberg equilibrium（P〉0.05）：（3） genetic variance＇ s effect on IMF content in Songliao black pig： HH was superior to Hh extreme significantly（P〈0.01） ,and Hh superior to hh extreme significantly（P〈0.01）, the least squares mean：2. 760〉2. 598〉2. 335 ,dd and Dd super to DD respectly extreme significantly（P〈 0.01 ）, the least squares mean： 2. 763 〉 2. 732 〉 2. 347, suggesting that Songliao black pig＇s meat quality could be improved by increasing HH-dd genotype frequency in porcine group.

关 键 词：猪 H-FABP基因 PCR-RFLP 肌内脂肪含量 

分 类 号：S815.8[农业科学—畜牧学]