低氧预适应小鼠皮层Bcl-2和Caspase-3的表达变化  

Changes in Bcl-2 and Caspase-3 expressions in cortex of hypoxic preconditioning mice

作　　者：张颜波[1] 吕国蔚[2] 杨明峰[1] 牛敬忠[1] 孙保亮[1] 

机构地区：[1]泰山医学院附属医院神经内科,泰安271000 [2]首都医科大学低氧医学研究所,北京100069

出　　处：《生理学报》2008年第2期249-253,共5页Acta Physiologica Sinica

摘　　要：本文旨在探讨小鼠皮层Bcl-2和Caspase-3在低氧预适应脑保护中的作用。将Blb/c近交系小鼠随机分为对照组、低氧组和低氧预适应组,用免疫荧光和激光共聚焦显微镜等技术测定皮层顶叶Bcl-2和Caspase-3表达荧光强度和阳性细胞计数。结果显示,低氧组和低氧预适应组Bcl-2表达均显著高于对照组,低氧预适应组又显著高于低氧组。低氧组和低氧预适应组Caspase-3表达均显著高于对照组,但低氧预适应组显著低于低氧组。结果表明,低氧预适应过程中,小鼠皮层脑区通过Bcl-2高表达和Caspase-3低表达抵御皮层细胞凋亡,从而参与脑保护机制。The purpose of the present study was to explore the roles of Bcl-2 and Caspase-3 in mouse cortex in hypoxic preconditioning. Blb/c mice were randomly divided into three groups： control group, hypoxic group and hypoxic preconditioning group. Huorescence intensity of Bcl-2 and Caspase-3 was observed and number of positive cells was counted in parietal cortex by inunanofluorescence and confocal laser scanning microscope. Huorescence intensity of Bcl-2 in the normal group, hypoxic group and hypoxic preconditioning group was 6.2± 1.7, 68.5± 13.1, 180.6±34.8, respectively, and number of Bcl-2-positive cells was 18.5±4.9, 52.3±10.5, 150.8±24.7, respectively. Huorescence intensity of Caspase-3 in the control group, hypoxic group and hypoxic preconditioning group was 8.6± 2.0, 40.2±8.2, 26.4±6.1, respectively, and number of Caspase-3-positive cells of was 4.3±1.2, 63.6±12.5, 45.7±9.8, respectively. The results showed that the expressions of Bcl-2 in both hypoxic group and hypoxic preconditioning group were significantly higher than that in the control group; and the expression of Bcl-2 in hypoxic preconditioning group was even higher than that in hypoxic group. The expressions of Caspase-3 in hypoxic group and hypoxic preconditioning group were also significantly higher than that in the control group; whereas the expression of Caspase-3 in hypoxic preconditioning group was significantly lower than that in hypoxic group. These results suggest that cortex cells are resistant to apoptosis via increased expression of Bcl-2 and lowered expression of Caspase-3 in the cortex and brain cells are thereby protected during hypoxic preconditioning.

关 键 词：低氧预适应 BCL-2 CASPASE-3 凋亡 皮层 小鼠 

分 类 号：R364.2[医药卫生—病理学]