Cross-talk between calcium-calmodulin and nitric oxide in abscisic acid signaling in leaves of maize plants  

作　　者：Jianrong Sang Aying Zhang Fan Lin Mingpu Tan Mingyi Jiang 

机构地区：[1]College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

出　　处：《Cell Research》2008年第5期577-588,共12页细胞研究（英文版）

基　　金：Acknowledgments This work was supported by the Major State Basic Research Program of China （grant no. 2003CB 114302 to M Jiang）, the National Natural Science Foundation of China （grant no. 30571122 to M Jiang）, and the Youth Scientific and Technological Innovation talent Project of Jiangsu Province （grant no. BK2007575 to A Zhang）.

摘　　要：Using pharmacological and biochemical approaches, the signaling pathways between hydrogen peroxide （H2O2）, calcium （Ca^2＋）-calmodulin （CAM）, and nitric oxide （NO） in abscisic acid （ABA）-induced antioxidant defense were investigated in leaves of maize （Zea mays L.） plants. Treatments with ABA, H2O2, and CaCl2 induced increases in the generation of NO in maize mesophyll cells and the activity of nitric oxide synthase （NOS） in the cytosolic and microsomal fractions of maize leaves. However, such increases were blocked by the pretreatments with Ca^2＋ inhibitors and CaM antagonists. Meanwhile, pretreatments with two NOS inhibitors also suppressed the Ca^2＋-induced increase in the production of NO. On the other hand, treatments with ABA and the NO donor sodium nitroprusside （SNP） also led to increases in the concentration of cytosolic Ca^2＋ in protoplasts of mesophyll cells and in the expression of calmodulin 1 （CaM1） gene and the contents of CaM in leaves of maize plants, and the increases induced by ABA were reduced by the pretreatments with a NO scavenger and a NOS inhibitor. Moreover, SNP-induced increases in the expression of the antioxidant genes superoxide dismutase 4 （SOD4）, cytosolic ascorbate peroxidase （cAPX）, and glutathione reductase 1 （GR1） and the activities of the chloroplastic and cytosolic antioxidant enzymes were arrested by the pretreatments with Ca^2＋ inhibitors and CaM antagonists. Our results suggest that Ca^2＋-CaM functions both upstream and downstream of NO production, which is mainly from NOS, in ABA- and H2O2-induced antioxidant defense in leaves of maize plants.

关 键 词：abscisic acid antioxidant defense CALMODULIN cytosolic calcium nitric oxide nitric oxide synthase Zea mays 

分 类 号：S513[农业科学—作物学]