Bromelain对HL-60细胞的诱导分化作用  

Differentiation of HL-60 cell induced by bromelain

作　　者：颜青[1,2] 杨善民[1,2] 朱林彬[1,2] 王重钦 周红[1,2] 

机构地区：[1]厦门大学抗癌研究中心 [2]厦门大学生物系

出　　处：《癌症》1997年第6期401-404,共4页Chinese Journal of Cancer

基　　金：福建省自然科学基金

摘　　要：目的：为获得对ＨＬ－６０细胞具有高效低毒作用的天然诱导分化剂而进行的研究。方法：以菠萝酶Ｂｒｏｍｅｌａｉｎ为药物，对人前髓细胞性白血病细胞株ＨＬ－６０进行作用，通过不同浓度及不同作用时间的药物作用，统计ＨＬ－６０细胞的增殖率、生化变化及形态学的变化。结果：发现Ｂｒｏｍｅｌａｉｎ在高浓度下（８μｇ／ｍｌ）作用４天即可诱导ＨＬ－６０细胞发生显著的诱导分化，其ＮＢＴ阳性率及形态学指标均达到６０％以上，电镜下核异染色质浓聚，出现特异性颗粒。结论：Ｂｒｏｍｅｌａｉｎ对ＨＬ－６０细胞具有明显的诱导分化作用，但其作用机理仍需进一步研究。Purpose: Is to select a differentiation inducer with high efficacy and low toxicity for HL-60 cells, a human promyelocyte leukemina cell line. Method: Bromelain was selected for this purpose. HL-60 cell was incubated with bromelain in a certain range of concentrations. The HL-60 cell growth, biochemical changes and morphological changes were determined. Results: both the NBT positive rate and the morphological changes rate were above 60% after induction of the cell with high concentration of Bromelain (8 μg/ml). Conclusion: Bromelain can induce HL-60 cell differentiation and reduce the malignant phenotypic characters of HL-60 cell. The mechanism of differentiation induction needs to be studied further.

关 键 词：BROMELAIN 诱导分化 蛋白水解酶 药物疗法 

分 类 号：R733.7[医药卫生—肿瘤] R73－361[医药卫生—临床医学]