阿蒂莫耶番荔枝总内酯体外抗肿瘤作用  

The cytotoxicity of the amnonaceous acetogenins in vitro

作　　者：符立梧[1,2] 潘启超[1,2] 陈文森 梁永钜[1,2] 

机构地区：[1]中山医科大学肿瘤研究所 [2]中国科学院华南植物研究所

出　　处：《癌症》1997年第6期409-410,共2页Chinese Journal of Cancer

摘　　要：目的：番荔枝内酯（Ａｍｎｏｎａｃｅｏｕｓａｃｅｔｏｇｅｎｉｎｓ）是从番荔枝科植物中得到的一类具有抗癌活性令人鼓舞的新型天然产物。同时其毒性低，活性高，极有希望成为新型的天然抗癌剂。阿蒂莫耶植物种子中番荔枝总内酯含有多种抗癌力强的成份，本文拟探讨这种番荔枝总内酯的体外抗肿瘤作用。方法：以阿蒂莫耶番荔枝内酯对多种人的体外培养的细胞株进行细胞毒测定。细胞毒测定方法为ＭＴＴ法。结果：阿蒂莫耶番荔枝内酯对人乳腺癌多药抗药性（Ｍｕｌｔｉｄｒｕｇｒｅｓｉｓｔａｎｃｅ，ＭＤＲ）细胞株ＭＣＦ－７／ＡＤＲ及敏感细胞株ＭＣＦ－７的ＩＣ５０分别为５．８３±１．４３及６．１０±１．０６μｇ／ｍｌ，对咽喉癌ＭＤＲ细胞株ＫＢｖ２００及敏感细胞株ＫＢ的ＩＣ５０分别为３．５８±０．８１及３．６４±０．７９μｇ／ｍｌ，对鼻咽癌ＣＮＥ２细胞株ＩＣ５０为２．５１±０．１１μｇ／ｍｌ。结论：阿蒂莫耶番荔枝内酯对不同肿瘤细胞系均具有很强的体外细胞毒活性。同时，ＭＤＲ细胞株对这种番荔枝总内酯无抗药性。Purpose: The total amnonaceous acetogenins (AA) was obtained from the seeds of Annona atemoya. It contains a lot of potent anticancer principles including bullatacin, atemoyacin-A, atemoyacin-B and so on. To develop AA as a new anticancer drugs, the cytotoxicities on human cancer cell lines was studied. Method: The cytotoxicities of AA were assayed by MTT method in various human cancer cell lines. Results: The IC50 of AA was 5.83±1.43 μg/ml for MCF-7 cells, 6.10±1.06 μg/ml for MCF-7/ADR cells, 3.64±0.79 μg/ml for KB cells, 3.58±0.81 μg/ml for KBv200 cells, 2.51±0.11 μg/ml for CNE2 cells, respectively. Conclusion :AA had potent cytotoxicity to various human cancer cell lines in vitro. There was no cross-resistance of AA to p-glycoprotein-positive MCF-7/ADR and KBv200 cell lines as compared to their sensitive parent cell lines.

关 键 词：总内酯 抗肿瘤作用 番荔枝内酯 阿蒂莫耶植物 

分 类 号：R73－361[医药卫生—肿瘤] R931.71[医药卫生—临床医学]