人肝细胞癌及癌旁肝组织HBV-DNA的原位杂交检测  

DETECTION OF HBVDNA IN HEPATOCELLULAR CARCINOMA AND PERICARCINOMATOUS TISSUES USING HYBIRDIZATION

作　　者：邹伟[1] 张昭成[1] 牛兆山[1] 

机构地区：[1]青岛医学院病理学教研室

出　　处：《青岛医学院学报》1997年第4期313-314,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省教委科技发展基金

摘　　要：①目的探讨乙型肝炎病毒（ＨＢＶ）ＤＮＡ与增生结节和肝癌的关系。②方法应用原位杂交技术检测５８例肝细胞癌（ＨＣＣ）及癌旁组织中ＨＢＶ－ＤＮＡ的表达。③结果肝细胞癌及癌旁组织中ＨＢＶ－ＤＮＡ阳性率分别为３７．９％（２２／５８）和４４．８％（２６／５８），差异无显著性（χ２＝０．５６，Ｐ＞０．０５）。ＨＢＶ－ＤＮＡ在癌旁组织中主要位于胞浆内，而肝癌组织中以胞核定位为主。２２例ＨＢＶ－ＤＮＡ阳性的ＨＣＣ中，１６例ＨＣＣ及癌旁组织均阳性，６例ＨＣＣ单独阳性，两者差异有显著性（χ２＝９．０９，Ｐ＜０．０１）。６例癌旁增生结节中ＨＢＶ－ＤＮＡ阳性（６／２７），以胞核定位为主。④结论ＨＢＶ感染与ＨＣＣ存在密切关系。癌周组织ＨＢＶ－ＤＮＡ以复制型为主，癌组织内ＨＢＶ－ＤＮＡ以整合型为主；癌旁增生结节为ＨＣＣ的癌前病变。Objective To study the relationship between HBVDNA and hepatocellular carcinoma (HCC),hyperplastic nodules. Methods Hybridization in situ method was used to detect HBVDNA in 58 cases of HCC and their surrounding tissues.Results The positive rate of HBVDNA in HCC and their surrounding tissues were 37.9%(22/58) and 44.8%(26/58) respectively. The difference was not signifciant(χ2=0.56,P>005). HBVDNA was mainly found in cytoplasm of pericarcinomatous tissues and in nucleus of HCC; Among the 22 HCC positive cases, 16 cases also showed pericarcinomatous tissue positive. Six cases(6/27) of hyperplastic nodules were nuclear HBVDNA positive. Conclusion HBV infection is a significant cause of HCC; HBVDNA in the pericarcinomatous tissue is of the replicative form, and in the HCC tissues, of the integrated form; hyperplastic nodule is the precancerous lesion of HCC.

关 键 词：肝肿瘤 脱氧核糖核酸 乙型肝炎病毒 原位杂交 

分 类 号：R735.702[医药卫生—肿瘤]