原位小肠移植后肠管吸收功能的研究  

Absorption of orthotopically transplanted small intestine in rats

作　　者：朱亮[1] 李幼生[2] 黎介寿[2] 

机构地区：[1]大连医科大学生理学教研室,116044 [2]南京军区南京总医院全军普外科研究所

出　　处：《中华实验外科杂志》2008年第5期612-614,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省医学领军人才课题[200710];辽宁省教育厅课题[2004F088];国家973计划资助项目[2003CB515502]

摘　　要：目的观察大鼠原位小肠移植后肠管的吸收功能的变化。方法60只Wistar大鼠随机分成两组，小肠移植组（采用改良的kort法行二步法大鼠原位小肠移植）和对照组，术后分别于2、4、8周检测移植小肠^15N—Gly的吸收和移植小肠功能酶（Na^＋，K＋^- ATP酶、双糖酶）活性。结果对^15N—Gly的吸收和小肠功能酶活性在原位移植术后2周下降明显，4周时双糖酶的功能、氨基酸的吸收恢复正常，8周时Na^＋ -K^＋ATP酶也接近正常。结论大鼠小肠原位移植后，移植小肠吸收功能在4周左右时接近正常水平。Objective To determine the changes in absorption of orthotopically transplanted small intestine in syngeneic rats. Methods Sixty Wistar rats were randomly divided into two groups （ n = 30） ： experimental group （The animals received two-step orthotopic intestine transplantation （OIT） using Kort＇s method modified by us） ,and control group. Separate experiments were performed 2,4 and 8 weeks after surgery to measure absorption of 15 N-Gly using stable isotope techniques in vivo and functional enzyme activity （disaccharides, Na^＋ /K^＋ -ATPase） in rat small intestinal epithelium. Results Compared with controls, 15 N-Gly absorption and intestinal functional enzyme activity were decreased at 2nd week after OIT. 15 N-Gly absorption and activity of disaccharides returned to baseline at 4th week in experimental group. In experimental group ,mucosal Na^＋/K^＋ -ATPase activity returned to baseline at 8th week. Conclusion The absorptive function of graft in rats is dose to the baseline at 4th week after OIT.

关 键 词：小肠移植 肠管吸收 酶活性 双糖酶 氨基酸 

分 类 号：R656.7[医药卫生—外科学] R446.112[医药卫生—临床医学]