原发和复发脑肿瘤干细胞在裸小鼠脑内侵袭性生长特征比较  

Comparison of two human brain tumor stem cell transplantation tumors in invasive growth feature ＇

作　　者：吴银艳[1] 刁艺[1] 黄强[1] 张全斌[1] 董军[1] 兰青[1] 

机构地区：[1]苏州大学附属第二医院神经外科,215004

出　　处：《中华实验外科杂志》2008年第5期617-618,682,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30400457、30672164、30772241）;江苏省自然科学基金资助项目（BK2007507）

摘　　要：目的观察人源脑肿瘤干细胞（hBTSC）在免疫缺陷动物脑内高致瘤性及高侵袭性。方法将原发和复发1×10^5个细胞的hBTSC球接种到40只裸小鼠脑内，移植瘤的组织学特点，免疫组织化学方法检测移植瘤中基质金属蛋白酶-1（MMP-1）的表达。结果40只裸小鼠在30d内全部成瘤。两种hBTSC在接种部位呈浸润性生长的同时，还散布于其他区域。但侵袭形式不尽相同，原发者在接种侧半球，呈团块，局限于脑实质；复发者，还浸润软脑膜和对侧半球。复发者MMPl＋细胞的分布比原发者密集。结论hBTSC除了高致瘤性，还具高侵袭性。复发hBTSC的侵袭性更强，可能与MMPl高表达有关。Objective To investigate the high oncogenicity and invasiveness of human brain tumor stem cell （hBTSC） transplantation tumor. Methods Primary and recurrent hBTSC spheres were injected into the brain of 40 nude mice. Histological characteristics and matrix metalloproteinase-1 （ MMP1 ） protein in the xenografts were determinated by HE staining and immunohistoehemical method, respectively. Results All 40 injected nude mice formed tumor in 30 days. Xenografts showed invasive growth in injected location and spread other area. The invision of primary and recurrent xenografts was different. Primary xenografts were concentrated in injected hemicerebrum. Recurrent xenografts displayed dissemination growth, even to reach hemicerebrum and cerebral pia mater of opposite side. MMP1 ＋ cells in primary xenografts were more than those in recurrent xenografts. Conclusion hBTSCs have strong oncogenicity and invasiveness. The stronger invasiveness of recurrent hBTSCs may be related to high expression of MMP1.

关 键 词：脑 肿瘤干细胞 致瘤性 侵袭性 

分 类 号：R739.4[医药卫生—肿瘤]