丙二醛、超氧化物歧化酶及肿瘤坏死因子-α在大鼠实验性胰性脑病中的作用  

Effects of MDA,SOD and TNF-α on The Experimental Models of Pancreatic Encephalopathy

作　　者：赵海平[1] 吕飞飞[1] 欧阳晓晖[1] 

机构地区：[1]内蒙古医学院附属医院普通外科,呼和浩特010050

出　　处：《中国普外基础与临床杂志》2008年第5期337-341,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：内蒙古自治区自然科学基金项目(编号:20050801909)~~

摘　　要：目的探讨丙二醛(MDA)、超氧化物歧化酶(SOD)及肿瘤坏死因子-α(TNF-α)在大鼠实验性胰性脑病中对脑组织的作用,为揭示胰性脑病的发病机理和治疗提供实验依据。方法将36只Wistar大鼠按拆信封法随机分为对照组(n=6)和胰性脑病组(n=30)。对照组经颈内动脉注入生理盐水;胰性脑病组经颈内动脉注入磷脂酶A2,复制大鼠胰性脑病模型。对照组大鼠于制模后第1d处死,胰性脑病组分别于造模后第1、3及7d各处死10只动物,检测脑组织含水量,观察脑微血管内白细胞聚集及附壁现象、脑细胞和神经纤维变化,检测大鼠脑组织匀浆中TNF-α、MDA含量及SOD活性变化。结果随时间延长,胰性脑病组大鼠脑微血管内白细胞聚集及附壁数目显著增加,神经元细胞水肿及神经纤维脱髓鞘改变明显,脑组织含水量、MDA及TNF-α的含量明显增加,SOD活性明显降低(P<0.05,P<0.01)。结论大鼠胰性脑病模型中,MDA、SOD及TNF-α对胰性脑病时脑损害的发生和发展有重要作用。Objective To study the effects of malondialdehyde （MDA）, superoxide dismutase （SOD） and tumor necrosis factor-or （TNF-α） on brain tissue in rats with pancreatic encephalopathy （PE）. Methods Thirty-six Wistar rats were randomly divided into control group （n=6） and PE model group （n= 30）. In control group, rats were injected with normal saline by internal carotid artery （0.1 ml/100 g） and were killed on the first day after the injection. In PE model group, rats were injected with phospholipases A2 （0.1 ml/100 g, 1 000 U/0.1 ml） by internal carotid artery, to establish animal model of PE in rat and 10 rats were killed on day 1, 3, 7 respectively after the injection. The changes of water content in the brain were measured. Leucocytes aggregation and margination in the microvessels, and the changes of cerebral cells and nerve fibers were observed. The levels of MDA, TNF-α and the activity of SOD were tested in the brain homogenate in rats. Results In PE model group, water contents of brain increased; The phenomena of leucocytes accumulation and margination, cellular edema of neurons and demyelination of nerve fibers became more obvious; The levels of MDA and TNF-α increased significantly than those in the control group, while the activity of SOD reduced （P〈0.05, P〈0.01）. Conclusion In the rat model of PE, MDA, SOD, and TNF-α play important roles on the occurrence and development of brain injury.

关 键 词：胰性脑病 大鼠 动物模型 丙二醛 超氧化物歧化酶 肿瘤坏死因子-Α 

分 类 号：R747.9[医药卫生—神经病学与精神病学]