水稻抗UV-B的QTL定位和环境互作分析  

QTL mapping and analysis of QTL×environment interaction associated with the resistance to enhanced UV-B radiation in rice(Oryza sativa L.)

作　　者：林文雄[1] 江宝月[1] 贾小丽[1] 邓家耀[1] 吴杏春[1] 梁康迳[1] 

机构地区：[1]福建农林大学农业生态研究所

出　　处：《中国生态农业学报》2008年第3期644-648,共5页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金项目(30571103,39970441);教育部生态学博士点基金项目(20050389004)资助

摘　　要：以“Lemont”（美国）和“Dular”（印度）杂交建立的包含123个家系的水稻重组自交系（RIL）群体，构建了含有97对SSR分子标记的水稻遗传连锁图谱。以该遗传群体及其亲本为材料，分别在2005年晚季和2006年早季进行UV—B辐射增强处理，考察了株高性状，并转换成抑制率进行混合线性模型的复合区间作图定位，共检测到2个抗UV—B辐射增强的加性QTLs，分别位于第4和第6染色体上，解释了4．72％和2．69％的遗传变异，分析还发现控制该性状QTL存在环境互作效应，分别解释了8．36％和5．42％的遗传变异，大于加性QTLs。同时检测到7对上位性互作基因，解释了0．00～6．88％的遗传变异，也存在-9环境的互作效应，解释了1．94％～23．31％的遗传变异，暗示着基因上位性的重要作用。We used a RIL （recombinant inbred line ） , which consisted of 123 family lines derived from a cross between ＂Lemont＂ and ＂Dular＂ associated with the resistance to enhanced UV-B radiation to construct a genetic linkage map by using 97 SSR makers. The RIL and its parents were grown under visible light with or without supplemental UV-B radiation in plastic pot in the late season of 2005 and the early season of 2006 respectively, to evaluate UV-B resistance by using the inhibitory rate of plant height （IPH） as an resistant trait. The genetic population of the RIL exhibited a wide range of variation in this trait. Using a mixed linear model approach with a LOD threshold of 2.0, two additive QTLs associated with the resistance to enhanced UV-B radiation were detected on chromosomes 4 and 6 respectively, explaining 4.72% and 2.69% of the total genetic variation respectively. It was also found that the two additive QTLs appeared to be significantly interacted with environment, accounting for 8. 36% and 5.42% of the total genetic variation, and the general contribution was 13.78% , which was larger than that of additive QTLs. Another 7 pairs of epistasis QTLs were detected, explainining 0.00 to 6.88% of the total genetic variation, which were also affected by environment, accounting for 1.94% - 23.31% of the total genetic variation respectively, implying that the digenic epistasis effect had a great role in resistance to enhanced UV-B radiation.

关 键 词：水稻 数量性状定位 重组自交系 抗UV—B 遗传连锁图谱 QTL与环境互作 

分 类 号：Q343.17[生物学—遗传学] X173[环境科学与工程—环境科学]