同种异体NK细胞对CD_(34)^+早期急性髓系白血病细胞的杀伤活性  

Cytotoxicity of allogenetic NK cells against CD_(34)^+ early acute myelogenous leukemia cells

作　　者：牛新清[1] 郭坤元[1] 周健[1] 胡海燕[1] 佘妙容[1] 

机构地区：[1]南方医科大学珠江医院,广东广州510282

出　　处：《山东医药》2008年第14期13-14,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30471636)。

摘　　要：目的探讨同种异体NK细胞对CD3+4早期急性髓系白血病(AML)细胞的体外杀伤活性。方法免疫磁珠法分离5例健康个体NK细胞,以NK杀伤敏感细胞株K562作为对照,乳酸脱氢酶(LDH)释放法测定不同效靶比时NK细胞对CD3+4早期AML细胞KG1a的杀伤活性。结果AML细胞株KG1a中CD34抗原表达率为98.0%±1.1%,分选后的NK细胞纯度为93.2%±3.7%。不同效靶比时NK细胞对KG1a细胞均有杀伤活性,且随着效靶比的增高,其杀伤活性增高(P<0.05)。结论同种异体NK细胞对CD34+早期AML细胞具有一定的杀伤活性。Objective To study cytotoxicity in vitro of aUogenetic NK cells on human CD34 early acute myelogenous leukemia cells. Methods Expression of CD34 antigen on acute myelogenous leukemia cell line-KGla cells was detected by FACS. Cytolysis against KGla cells of NK cells isolated from 5 healthy persons with MACS was detected by LDH releasing assay at different effect-to-target （ E： T） ratios, compared with K562 which was sensitive to NK cells. Results Expression of CD34 antigen on KGla cells was 98.0% ± 1.1% and purity of NK cells after isolated was 93.2% ±3.7% detected by FACS. There was cytotoxiclty in vitro of aUogenetic NK cells against KGla cells at different E： T ratios. With increasing of E： T ratios, cytotoxicity of allogenetic NK cells against KGla cells was increased （P 〈 0. 05）. Conclusion Allogenetic NK cells display cytotoxicity against CD34^＋ early acute myelogenons leukemia cells.

关 键 词：杀伤细胞 天然 白血病 髓样 杀伤活性 

分 类 号：R733.71[医药卫生—肿瘤]