鼻咽癌细胞株裸鼠肝异位种植瘤肺转移动物模型的建立  

Establishment of the Liver Heterotopic Transplantation and Lung Metastasis Model of Human Nasopharyngeal Carcinoma in Nude Mice

作　　者：李晓华[1] 詹志荣[1] 孙建设[1] 曾斌[1] 

机构地区：[1]中国人民解放军第九四医院耳鼻咽喉科,南昌330002

出　　处：《江西医学院学报》2008年第2期32-33,37,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的建立鼻咽癌细胞株肝异位种植瘤肺转移裸鼠的动物模型。方法24只裸鼠分别通过门静脉(门静脉组)或肝包膜下(肝包膜组)注射鼻咽癌细胞株C666-1细胞悬液,建立鼻咽癌细胞株肝异位种植瘤肺转移裸鼠动物模型,3周后每组随机处死6只裸鼠,观察裸鼠腹水、肝脏成瘤、肺转移等情况,其余裸鼠观察其生存期。结果门静脉组和肝包膜组肝脏成瘤率、腹水形成率均为100%,肺转移率分别为66.7%和58.3%,两组经卡方检验差异无显著意义(P>0.05)。平均生存期分别为(25.17±5.15)d及(22.50±3.89)d,经log-rank时序检验差异无显著性意义(P>0.05)。结论两种方法均能成功建立鼻咽癌细胞株肝种植瘤肺转移裸鼠的动物模型。Objective To establish the liver heterotopic transplantation and lung metastasis model of human nasopharyngeal carcinoma in nude mice. Methods Twenty-four nude mice were divided into 2 groups randomly. The human nasopharyngeal carcinoma cells C666-1 were injected into the mice liver directly （liver capsule group） or via portal vein （portal vein group）. After three weeks, six mice from each group were chosen randomly and were sacrificed. Tumorigenecity and metastasis were evaluated subsequently. The remainders were left to die naturally to evaluate the long-term survival. Results Both the rate of the tumor formation in liver and the rate of the ascites formation in two groups were 100%. The rates of lung metastases in portal vein group and liver capsule group were 66.7% and 58.3%,respectively（P〉0.05）. The survival time of tumor bearing mice was （25. 17±5.15）d and （22. 50±3.89）d respectively（P〉0.05）. ConclusionThe liver heterotopic transplantation and lung metastasis model of human nasopharyngeal carcinoma in nude mice is established successfully by injecting C666-1 into the mice liver directly or via portal vein.

关 键 词：鼻咽肿瘤 动物模型 肿瘤转移 裸鼠 

分 类 号：R739.63[医药卫生—肿瘤]