末端限制性酶切片段长度多态性分析一个能够降解聚乙烯醇的混合体系  

A Poly(vinyl alcohol)-Degrading Mixed Culture Analyzed by Terminal Restriction Fragment Length Polymorphism

作　　者：张颖[1] 堵国成[2,3] 刘和[2,3] 李光伟[2,3] 陈坚[2,3] 

机构地区：[1]江南大学生态纺织教育部重点实验室,江苏无锡214122 [2]食品科学与技术国家重点实验室,江南大学,江苏无锡214122 [3]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与生物技术学报》2008年第1期84-89,共6页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2003AA322050);江苏省高技术项目(BG2005016);新世纪优秀人才支持计划项目

摘　　要：用末端限制性酶切片段长度多态性(Terminal restriction fragment length polymorphism,T-RFLP)技术在DNA水平分析了一个能够彻底降解1 g/L聚乙烯醇(PVA)1799的混合微生物体系在降解PVA过程中,混合体系的微生物种类及数量的变化。并用高效液相法(HPLC)对该混合体系降解PVA1799的中间产物进行了初步研究,发现在降解过程中有乙酸及甲基酮类物质生成,提出了该混合体系降解PVA1799的可能途径。The dynamic composition of species and quantity in a mixed culture, which was capable of completely degrading 1 g/L poly （vinyl alcohol） （PVA） 1799, was investigated usingTerminal Restriction Fragment Length Polymorphism （T- RFLP） technology during the degradation process. The process of PVA degradation by the mixed culture was investigated using HPLC, etc. Besides the extracellular PVA-degrading enzymes, the bacterial cells also played a very important role in PVA degradation. Small PVA molecules were found in the cell extract. Acetic acid and methyl-ketone substance were produced during the degradation process. A possible pathway was proposed for PVA degradation by the mixed culture.

关 键 词：聚乙烯醇(PVA) 混合体系 生物降解 

分 类 号：Q93[生物学—微生物学]