CK(AE1/AE3)在喉鳞癌淋巴结微转移诊断中的应用  

Significance of CK(AE1/AE3) in detecting lymph node micrometastases in patients with laryngeal squamous carcinoma

作　　者：田俊[1] 王斌全[1] 皇甫辉[1] 李建明[2] 温树信[1] 

机构地区：[1]山西医科大学第一医院耳鼻咽喉科,山西太原030001 [2]山西省肿瘤医院

出　　处：《中国现代医药杂志》2008年第5期27-29,共3页Modern Medicine Journal of China

摘　　要：目的研究细胞角蛋白广谱抗体CK(AE1/AE3)作为免疫标志物在喉鳞癌淋巴结微转移诊断中的应用与临床病理意义。方法对50例喉鳞癌患者喉标本及其常规病理报告为阳性的140个淋巴结和阴性的756个淋巴结重新切片,以CK(AE1/AE3)作为免疫标志物,采用免疫组化PV9000两步法检测。结果在50例喉鳞癌原发灶和病理检查阳性淋巴结中,CKAE1/AE3全部表达,和临床T分期、病理分级无明显相关(P>0.05),756个阴性淋巴结标本中,有9例(18.0%)16个淋巴结(2.1%)发现了微转移灶。结论CK(AE1/AE3)免疫组化法是检测喉鳞癌淋巴结转移的敏感而便捷的方法,特别对筛选组织学检查淋巴结阴性但存在微转移的患者有一定实用价值。Objective To evaluate the significance of antibody Cytokeratin pan （CK AE1/AE3） in diagnosing micrometastases in lymph nodes in patients with laryngeal squamous carcinoma. Methods Fifty cases of laryngeal squamous carcinoma together with 140 pathologically positive lymph nodes and 756 negative ones were studied by immunostaining with antibody CK（AE1/AE3） on histological sections of the primary tumor and regional lymph nodes. Results In all cases,CK（AE1/AE3） was positively expressed in the primary tumor and 140 pathologically positive lymph nodes.It did not display an obvious correlation with T staging and pathological grading （P〉0.05）. Among 756 pathologically negative lymph nodes,metastases were confirmed in 16 lymph nodes from 9 cases. Conclusion Immunohistochemical staining with antibody CK（AE1/AE3） is a sensitive and convenient method for the detection of node micrometastases in patients with laryngeal squamous carcinoma.

关 键 词：喉肿瘤 淋巴转移 角蛋白 免疫组织化学 

分 类 号：R739.6[医药卫生—肿瘤]