反义c-myc寡核苷酸对hTERT蛋白表达抑制作用的研究  

A Study of the Liposome-Mediated c-myc Antisense Oligonucleotide Inhibit hTERT Protein Expression of MCF-7

作　　者：彭春[1] 税青林[2] 赵小平[2] 张莉娟[2] 马莉[2] 

机构地区：[1]焦作市人民医院病理科,河南焦作454000 [2]泸州医学院生物学与遗传学教研室,四川泸州646000

出　　处：《肿瘤基础与临床》2008年第3期214-216,共3页journal of basic and clinical oncology

基　　金：四川省教育厅自然基金重点资助项目(编号:2002A068)

摘　　要：目的探讨脂质体LipfectAMINETM(LR)介导的反义c-myc寡核苷酸(ASODN)对乳腺癌MCF-7细胞hTERT蛋白表达的抑制作用。方法应用免疫细胞化学ABC法检测转染反义、正义及未转染细胞中hTERT蛋白的表达情况。结果免疫细胞化学显示转染反义、正义与未转染细胞的hTERT蛋白表达分别为23%、96%、99%(P<0.01),表明转染反义c-myc的MCF-7细胞hTERT蛋白表达明显降低。结论反义c-myc寡核苷酸可以抑制hTERT蛋白的表达。Objective To investigate the inhibitive effect of hTERT protein expression of MCF-7 by liposome（ LipfectAMINETM , LR）-mediated c-myc antisense oligonucleotide. Methods hTERT protein expressions of the transfected or untransfected cells were detected by immunocytochemistry method. Results Immunocytochemistry showed that hTERT protein expressions in transfected cells were obviously de- creased after being treated for 72 h. The positive rate of hTERT protein expressions of the cells of untransfected, transfected SO or transfected AS group were 23% , 96% , 99% respectively. Conclusions c-myc ASODN could inhibit hTERT protein expressions in MCF-7 cells.

关 键 词：反义c—myc寡核苷酸 MCF-7细胞 HTERT基因 免疫细胞化学 

分 类 号：R737.9[医药卫生—肿瘤]