免疫细胞化学在胸腔积液转移癌与恶性间皮瘤鉴别诊断中的应用  

The Application of Immunocytochemistry Markers in Cytological Differential Diagnosis of Malignant Pleural Effusion

作　　者：许艳梅[1] 黄庆玉[1] 王家富[1] 刘蕾[1] 

机构地区：[1]泰山医学院病理教研室,山东泰安271000

出　　处：《肿瘤基础与临床》2008年第3期223-225,共3页journal of basic and clinical oncology

摘　　要：目的探讨免疫细胞化学特异性标记转移性腺癌及恶性间皮瘤细胞在恶性胸腔积液细胞学鉴别诊断中的意义。方法收集恶性胸腔积液标本52例,免疫细胞化学S-P法标记一组单克隆抗体:CEA、TAG-72、CD15、Calretin in、MC。结果上述几种抗体在转移癌和恶性间皮瘤中的表达差异均有统计学意义。联合检测两种抗体可以提高诊断的敏感性,CD15与TAG-72联合检测的敏感性为83.7%。结论选择一组特异性免疫细胞化学标记物,是对传统的细胞形态学检查的必要补充,对恶性胸腔积液中转移癌和恶性间皮瘤的鉴别诊断起重要作用。Objective To explore the significance of immunocytochemistry in the differential diagnosis of metastatic adenocarcinoma and ma- lignant mesothelioma in pleural effusion. Methods Immunoeytochemistry S-P labeling of a monoclonal antibodies CEA, TAG-72, CD15, Calretinin, and HBME-1 in 52 cases with malignant pleural effusion. Results Antibody response in metastatic adenocarcinoma and malignant mesothelioma was statistically significant. Combined detection of the two antibodies could enhance the sensitivity of diagnosis with CD15 and TAG-72 joint detection sensitivity of 83.7% . Conclusions Using a specific group of immunocellular markers can play an important role in the differential diagnosis of metastatic adenocarcinoma and malignant mesothelioma in malignant pleural effusion.

关 键 词：免疫细胞化学 胸腔积液 腺癌 恶性间皮瘤 诊断 

分 类 号：R730.43[医药卫生—肿瘤]