CIK细胞治疗中晚期消化系统肿瘤的临床研究  

A Clinical Study of the Treatment of Patients with Advanced Digestive Malignant Tumor(DMT) with CIK Cells

作　　者：刘继斌[1] 施玲燕[1] 刘晓玲[1] 王强[1] 郁惠高[1] 沈康[1] 张一心[1] 

机构地区：[1]南通市肿瘤医院生物中心,江苏南通226361

出　　处：《肿瘤基础与临床》2008年第3期252-254,共3页journal of basic and clinical oncology

摘　　要：目的评估细胞因子诱导的杀伤细胞(CIK细胞)过继免疫疗法治疗中晚期消化系肿瘤的临床疗效。方法参照美国斯坦福大学骨髓移植中心建立的CIK细胞培养方法诱导扩增CIK细胞。培养14 d后分次回输给患者,观察患者治疗前后瘤体变化、肿瘤标志物变化、免疫指标变化、临床症状改善、生活质量、卡氏评分等,同时记录生存期。结果在53例接受CIK细胞治疗中晚期消化系肿瘤患者中,PR+MR为39例,总缓解率为73.6%,随访1年生存率为92.4%,2年为83.0%,3年为75.5%。临床症状治疗前后有明显改善(P<0.05);生存质量卡氏评分提高率为79.24%;治疗前后CD4、CD19、CD3/CD16+CD56差异均有统计学意义(P<0.05);肿瘤标志物CA199差异有统计学意义(t=7.91P<0.05);体重多数有增加。结论CIK细胞对手术、放疗、化疗已无适应证的中晚期消化系肿瘤患者提供了一种可以延长生存期、提高生活质量的新途径。Objective To estimate the clinical effects on patients with advanced digestive malignant tumor （DMT） with cytokine-induced killer cells （CIK）. Methods CIK cells were induced by culturing PBMC of healthy people by incubation in vitro with IL-2.γ-IFN.anti-CD3 monoclonal antibody and IL-1, then were transfused back to the patients with advanced digestive malignant tumor after two weeks, for observation the changes of tumor weight, life quality, immunity indexes, tumor markers （TM） and the Karnofsky＇ s grade. Results The effective rate of CIK was 73.6% （39/53） ,after follow-up 3 years, survival period extension, survival rate was 92.4% after 1 year, after 2 year was 83.0% ,and after 3 year was 75.5%. All the patients had no apparent side effect. The CA199 from patients with DMT after transfused was significantly lower than that before transfused （ t = 7.91, P 〈 0.05 ） , The CD4, CD19, CD3/CD16 - CD56 from patients with DMT after transfused were significantly higher than that before transfused （P 〈 0.05 ） , the Karnofsky＇ s grade and survival period after transfused CIK were significantly superior to that before transfused. Conclusions CIK cells therapy can enhance effect of the anti-cancer, improve survival quantity and prolong survival period. , which probably can provide a potent immune therapeutic strategy for patients with advanced digestive malignant tumor.

关 键 词：中晚期消化系肿瘤 CIK细胞 过继免疫疗法 

分 类 号：R730.51[医药卫生—肿瘤] R735[医药卫生—临床医学]