^60Coγ射线照射人肝细胞传40代后的差异表达基因谱的变化  

DIFFERENTIAL EXPRESSION OF GENOME IN THE PROGENY DOUBLING 40 GENERATIONS OF NORMAL HUMAN LIVER CELLS IRRADIATED BY ^(60)Co γ RAYS

作　　者：左雅慧[1] 王放[1] 耿晓华[1] 王小莉[1] 李建国[1] 王仲文[1] 童建[2] 

机构地区：[1]中国辐射防护研究院,太原 030006 [2]苏州大学放射医学与公共卫生学院,215123

出　　处：《辐射防护》2008年第3期170-176,共7页Radiation Protection

基　　金：国防基础科研资助项目

摘　　要：利用基因芯片技术比较经4Gy和8Gy60Coγ射线照射后传40代的人正常肝细胞子代克隆的基因表达图谱,探讨辐射对人肝细胞基因表达的影响;利用实时荧光定量RT-PCR技术对受照细胞子代两个共有的差异表达基因VTN、INSIG1进行验证。结果显示,4Gy照射后子代细胞差异表达显著的基因有58条;8Gy照射后子代细胞差异表达显著的基因有882条;4Gy和8Gy共同差异表达的基因有16条;差异表达的基因与照射剂量有相关性。这些差异基因的功能涉及到免疫、信号转导、细胞周期调控、代谢等。RT-PCR检测结果与基因芯片结果一致。结果表明不同剂量的照射可导致人肝细胞基因组的差异表达,该结果为从分子水平上探讨辐射诱发的基因组不稳定性提供基础资料。In order to investigate, the differential expression of genome in nomal human liver cell irradiated by ^60Coγ rays, cDNA chip was applied to assay the alterations of transcriptional profile of nomal human liver cell＇progeny exposed to 4 Gy and 8 Gy of 7 ray from ^60Co. Then Quantitative real-time PCR was used to confirm the commonly changed genes including VTN and INSIG1. The results demonstrated that the transcription level of 58 genes changed in the progeny of survival ceils irradiated by 4 Gy γ rays, while 882 genes changed in the progeny of survival cells irradiated by 8 Gy γ rays. 16 genes changed in these cells irradiated by both 4 and 8 Gy γ rays. The transcription level was correlated to the original irradiation dose. Most of the genes were associated with transduction, cell cycle regulation, cellular immunity, cytoskeleton and movement, cell replication and repair mechanism, etc. The changed transcriptional level was further confirmed by RT-PCR assay for some of these genes. The results showed that differential expression of genome irradiated by different doses of γ rays. These results offer the fundamental materials for the research of genomic instability induced by radiation on the molecular level.

关 键 词：基因芯片 基因差异表达 电离辐射 基因组不稳定性 

分 类 号：Q3[生物学—遗传学]