重组人p53腺病毒注射液联合放射治疗对人淋巴瘤细胞的影响  

EFFECT OF RECOMBINANT ADENOVIRUS ENCODING HUMAN p53 TUMOR SUPPRESSOR GENE COMBINED WITH RADIATION THERAPY ON HUMAN LYMPHOMA CELLS LINES

作　　者：俞泽阳[1] 范我[1] 吴锦昌[2] 李栋庆[3] 朱然[3] 万建美[3] 王永青[3] 

机构地区：[1]苏州大学放射医学与公共卫生学院,215123 [2]南京医科大学附属苏州市立医院,215001 [3]苏州大学第二附属医院,215004

出　　处：《辐射防护》2008年第3期177-183,共7页Radiation Protection

基　　金：国家自然科学基金资助项目(项目编号:30471974)

摘　　要：为探讨重组人p53腺病毒(rAd-p53)注射液联合放射治疗对人淋巴瘤细胞的抑制作用及其辐射增敏性,利用四氮唑盐(MTT)比色法分析rAd-p53注射液、放射治疗以及两者联合应用对人淋巴瘤细胞的抑制作用;应用流式细胞术分析各组细胞的细胞周期和凋亡率;通过蛋白质的Western印迹分析法(Western-blotting)分析各组淋巴瘤细胞内P53蛋白的表达。结果表明外源性的p53基因虽然有一定程度的转染,但未见高效表达,rAd-p53注射液对人淋巴瘤细胞的抑制作用及辐射增敏性均不显著。This paper analyzes the inhibitory effect and radiation sensitization of recombinant adenovirus encoding hmnan p53 tumor suppressor gene （rAd-p53） on hmnan lymphoma cell lines. Hmnan lymphoma cell lines were treated with rAd-p53, radiation therapy and combined treatment, respectively. The cell growth inhibition was assessed by MTT. The cell cycle and apoptosis were detected by flow cytometry, and the p53 protein expression was detected by Westem blotting. The results showed that extrinsic p53 gene have expressed to some degree, but not at high level. The role of inhibition and radiation sensitivity of rAd-p53 was not significant to human lymphoma cell lines.

关 键 词：重组人P53腺病毒注射液 P53基因 人淋巴瘤细胞 放射治疗 

分 类 号：R733.1[医药卫生—肿瘤]