Survivin反义寡核苷酸增强U251人胶质瘤细胞对顺铂的敏感性研究  

Survivin gene antisense Oligodeoxynucleotide enhances sensitivity of glioma cell U251 to Cisplatin in vitro

作　　者：成志[1] 阳纲要[1] 

机构地区：[1]南华大学附属第一医院神经内科,湖南衡阳421001

出　　处：《中国现代医学杂志》2008年第9期1187-1189,1193,共4页China Journal of Modern Medicine

摘　　要：目的研究Survivin反义寡核苷酸对U251人胶质瘤细胞顺铂治疗敏感性的影响。方法脂质体介导Survivin反义寡核苷酸转染U251人胶质瘤细胞,Western blot法检测内源性Survivin表达水平的变化,MTT法检测顺铂对细胞生长情况的影响,流式细胞仪(FCM)观察顺铂诱导各组U251细胞的凋亡。结果反义Survivin脂质体复合物能有效下调Survivin表达水平,并且能抑制U251细胞的生长,提高U251细胞对顺铂的敏感性,流式细胞仪检测凋亡率明显提高。结论Survivin反义寡核苷酸能增强顺铂诱导的U251细胞凋亡,Survivin反义寡核苷酸和顺铂联合用药对U251细胞生存具有协同抑制效应,可改善治疗效果。[Objective] To assess the effects of Survivin antisense Oligodexynucleodides （ASODN） on chemosensitivity of brain glioma cell U251 to Cisplatin in vitro. [Method] The glioma cells U251 transferred with Survivin ASODN for 48h. Expression of endogenous Survivin protein was determined by Western blot. Cell Counting and MTT assay were performed to evaluate the sensibility of transferred cells to Cisplatin. The rate of apoptosis was tested by FCM. [Results] Transfection of the Survivin ASODN can inhibit the expression of endogenous Survivin protein and inhibit the growth of U251 cells. The sensitivity of U251 cells to Cisplatin was enhanced after transfection. The rate of apotosis increased after Survivin ASODN was transferred into U251 cells. [Conclusion] Survivin ASODN could enhance cisplatin-induced apotosis in U251 cells. Combination of Survivin ASODN and Cisplatin could improve the treatment effect on brain glioma.

关 键 词：SURVIVIN 寡核苷酸类 人胶质瘤细胞 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]