慢性和慢性重型乙型肝炎患者外周血单核细胞TOLL样受体2的变化及其与白细胞介素6的关系  

Correlation between Toll-like receptor 2 on peripheral blood monocytes and serum IL-6 in patients with chronic and chronic severe hepatitis B

作　　者：尉秀清[1] 文卓夫[1] 郑丰平[1] 姚集鲁[2] 

机构地区：[1]中山大学附属第三医院消化内科,广东广州510630 [2]中山大学附属第三医院感染科,广东广州510630

出　　处：《中国现代医学杂志》2008年第9期1201-1203,1207,共4页China Journal of Modern Medicine

摘　　要：目的探讨慢性乙型肝炎和慢性重型乙型肝炎患者外周血单核细胞TOLL样受体2(TLR2)的变化情况及其意义。方法用流式细胞仪检测30例正常对照者、31例慢性乙型肝炎患者和30例慢性重型乙型肝炎患者外周血单核细胞表面TOLL样受体2的表达,ELISA法检测上述患者血清白细胞介素6(IL-6)的水平。结果正常对照组、慢性乙型肝炎组和慢性重型乙型肝炎组外周血单核细胞TLR2的平均免疫荧光强度和外周血清IL-6水平分别为(21.5±2.7)、(39.0±4.1)、(47.7±21.4)MFI和(11.5±7.2)、(40.8±31.2)、(77.6±33.3)ng/L,自正常对照组到慢性乙型肝炎组及慢性重型乙型肝炎组外周血单核细胞TLR2表达强度和外周血清IL-6水平均依次升高,且各组间比较差异均具有显著性(P<0.05);外周血单核细胞TLR2表达水平与血清IL-6表达水平呈显著正相关,相关系数γ=0.769,P<0.05。结论TLR2可能与慢性乙型肝炎及慢性重型乙型肝炎的发病有关。[Objective] To study the change of TLR2 on peripheral blood monocytes （PBMCs） and its role in the pathogenesis of chronic hepatitis B and chronic severe hepatitis B. [Methods] The expression of TLR2 on 10000 CD14＋ PBMCs was determined by flow cytometer in 30 healthy controls, 31 patients with chronic hepatitis B and 30 patients with chronic severe hepatitis B. The level of serum interleukin-6（IL-6） was determined by ELISA. [Results] The values of TLR2 on PBMCs and serum IL-6 of the groups of healthy control, patients with chronic hepatitis B and patients with chronic severe hepatitis B were （21.5±2.7）, （39.0±4.1）, （47.7±21.4） mean fluorescence intensity （MFI） and （11.5±7.2）, （40.8±31.2）, （77.6±33.3） ng/L. Both of them increased gradually and significantly from the group of healthy control to the group of patients with chronic hepatitis B and patients with chronic severe hepatitis B. There was a significant positive correlation between the value of TLR2 and the value of serum IL-6. [Conclusion] There may be a role of TLR2 in the pathogenesis of chronic hepatitis B and chronic severe hepatitis B.

关 键 词：乙型肝炎 TOLL样受体2 白细胞介素6 

分 类 号：R575.1[医药卫生—消化系统]