脑缺血再灌注后大鼠IGF-1及其受体表达对其神经行为的影响  

EFFECTS OF IGF-1 AND IGF-1R ON NEURO BEHAVIOR AFTER CEREBRAL ISCHEMIC REPERFUSION IN RATS

作　　者：孙锋[1] 刘蔚[2] 丁晓洁[1] 王超[1] 郭云良[3] 

机构地区：[1]青岛大学医学院第二附属医院神经科,山东青岛266042 [2]青岛大学医学院第二附属医院查体中心 [3]青岛大学医学院脑血管病研究所

出　　处：《青岛大学医学院学报》2008年第2期123-125,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的观察脑缺血再灌注后大鼠胰岛素生长因子-1(IGF-1)及其受体(IGF-1R)表达对其神经行为的影响。方法成年健康雄性Wistar大鼠28只,应用线栓法经左侧颈外-内动脉插线建立大脑中动脉闭塞再灌注(MCAO/R)模型。应用BEDERSON评分法评价脑缺血再灌注后神经行为功能,免疫组织化学技术检测IGF-1和IGF-1R表达。结果脑缺血再灌注后各组动物均出现不同程度的神经功能受损,从再灌注3 d开始神经功能逐渐恢复,但至再灌注7 d才有显著性差异。免疫组化检测显示,脑缺血再灌注后脑组织IGF-1和IGF-1R表达区域和时相变化规律基本一致,再灌注12 h^7 d持续高表达,1~3 d达高峰。结论脑缺血再灌注后IGF-1和IGF-1R的早期表达可能对神经功能恢复有一定的促进作用。Objective To investigate the effects of insulin-like growth factor （IGF-1） and its receptor （IGF-1R） on neurobehavior after cerebral isehemie reperfusion in rats. Methods A middle cerebral artery oeelusion/reperfusion （MCAO/R） model was made in 28 healthy male adult Wistar rats, the neurobehavior of these rats was evaluated via Bederson,s score, and expressions of IGF-1 and IGF-1R were determined by immunohistoehemieal assay. Results All the MCAO/R animals appearred different extent of neurofunetional impairment. Recovery started from the 3rd day after reperfusion, its significant improvement on 7th day. The expressions of IGF-1 and IGF-1R lasted for 12 h-7 days after reperfusion, reached its peak for 1-3 days, which was ba sieally consistent in area and time-phase. Conclusion The early expressions of IGF-1 and IGF-1R might enhance the neurofunetional recovery after cerebral isehemie reperfusion.

关 键 词：脑缺血 神经行为功能 胰岛素生长因子-1 胰岛素生长因子-1受体 

分 类 号：R743.3[医药卫生—神经病学与精神病学]