兔肝硬化肝癌与单纯肝癌立体定向照射后病理形态学对比分析  

Changes of Pathological Morphology in Liver Cirrhosis and Hepatocarcinoma of Rabbits after Stereotactic Irradiation

作　　者：丁轶[1] 陈斌[2] 陈龙华[1] 

机构地区：[1]南方医科大学南方医院放疗科,广州510515 [2]南方医科大学南方医院肿瘤科,广州510515

出　　处：《肿瘤防治研究》2008年第5期305-308,共4页Cancer Research on Prevention and Treatment

基　　金：广东省自然科学基金资助项目(2005004776)

摘　　要：目的应用已制备的兔肝硬化肝癌模型和注射VX2肝癌细胞制备的转移癌模型对比观察肝癌照射后病理形态学的变化。方法肝硬化肝癌兔16只(实验组1),随机分为两组,每组8只,分别给予单次剂量20、30Gy的立体定向照射;8只单纯接种肝癌兔(实验组2),随机分为两组,每组4只,按上述方法给予相同照射。照射后3周处死实验组1与实验组2,应用HE染色比较照射前后实验组1与实验组2肝脏病理形态学变化;用网状纤维染色、Masson三色比较和确定照射前后实验组1与实验组2肝脏纤维化程度。结果照射后肿瘤区瘤细胞大片坏死,间质纤维组织增生肿胀,部分纤维组织断裂。实验组1瘤组织周围的肝细胞坏死较明显;实验组2瘤组织周边肝细胞以变性为主。照射前后网状纤维及Masson三色:实验组1照射后与照射前相比,周围纤维组织断裂;实验组2照射前后纤维组织无明显改变。结论肝病状态下肝硬化肝癌的放射治疗与正常状态下肝癌相比,肝脏耐受剂量明显降低。Objective Using hepatocarcinoma model of rabbits based on liver cirrhosis and injected VX2 cells to observe changes of pathological morphology after irradiation under state of liver disease. Methods Sixteen rabbits with liver cirrhosis and hepatocarcinoma （experimental group one）, and those were randomly divided into two groups（n = 8）. Eight rabbits with hepatocarcinoma（experimental group two）, and those were also randomly divided into two groups（n = 4）. The stereotactic irradiation with sin- gle dose of 20 or 30 Gy was respectively performed on each experimental group. Rabits of experimental groups were both killed after irradiation for three weeks. The liver pathological morphological changes in the experimental groups one and two were observed by pathological routine examination before and after irradiation. Reticular fiber and Masson＇s trichrome stainings were performed to analyze and identify the difference of liver fibrosis between the two groups before and after irradiation. Results Massive necrosis of tumor tissues were observed and mesenchymal fibrous tissues were swelled and partial disrupted in experimental groups. In experimental group one, obvious necrosis of hepatocytes was seen around the tumor tissues, with slightly segmentation. In experimental group two,most of hepatocytes around the tumor tis- sues were degenerated, and there were no marked changes in fibrous tissue in experimental groups through observation of reticular fiber and Masson stain before and after irradiation. Conclusion The liver tolerance obviously depress after irradiation for hepatocarcinoma based on liver cirrhosis.

关 键 词：肝硬化 肝癌 立体定向照射 网状纤维染色 Masson三色 HE染色 

分 类 号：R735.7[医药卫生—肿瘤]