聚L-苏氨酸铅笔芯修饰电极对盐酸异丙嗪的电化学行为及测定研究  

Study on the Electrochemical Behaviors and Determination of Promethazine at poly(L-threomine) Lead Modified Electrode

作　　者：李利军[1] 喻来波[2] 程昊[1] 陈其锋[2] 吴峰敏[2] 朱晓勇[2] 吴健玲[2] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]广西大学化学与化学工程学院,广西南宁530004

出　　处：《分析测试学报》2008年第5期517-522,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20665001);广西科学基金资助项目(0640029);广西工学院硕士基金资助项目(500527)

摘　　要：通过循环伏安法（CV法）在铅笔芯电极上成功地制备了聚L-苏氨酸修饰膜，研究了铅笔芯修饰电极上的最佳聚合条件，并对电极的表面结构进行显微表征。同时，研究了盐酸异丙嗪在该修饰电极上的电化学行为。由盐酸异丙嗪在聚L-苏氨酸修饰电极上的氧化和高锰酸钾在金电极上的还原组成双安培检测体系，建立了在外加电压为0V条件下流动注射双安培法直接测定盐酸异丙嗪的新方法。在pH6．8PBs（磷酸盐缓冲溶液）中，该氧化峰峰电流与盐酸异丙嗪浓度在2．0×10^-6～1．5×10^-3mol／L范围内呈线性关系（r=0．9979，n=12），线性回归方程为，（nA）=1．97×10^7c-300，方法检出限为8．5×10^-7mol／L（S／N=3）。RSD为1．60％（n=20），进样频率为100样／h。The poly（L-threonine） modified electrode was prepared on the lead electrode by using the cyclic vohammetry and the optimum polymerization conditions were studied, The surface configuration of the modified electrode was characterized by the microscope. The electrochemical behaviors of promethazine at the poly（L-threonine） lead modified electrode were studied. By using the flow injection irreversible biamperometric system in which the oxidation of promethazine took place at the L- threomine lead modified electrode and the reduction of potassium permanganate at the gold electrode, a method was established for the direct determination of promethazine under a potential difference of 0 V, In a phosphate buffer solution（PBS） of pH 6, 8, the oxidative peak current increased linearly with the promethazine concentration in the range of 2.0 × 10^-6 - 1.5 ×10^-3 mol/L with a sampling frequency of 100 samples per hour, The detection limit for promethazine is 8.5 × 10^ -7 mol/L and the RSD for 20 replicate determinations of 2.5 × 10^ -5 mol/L promethazine is 1.60%.

关 键 词：聚L-苏氨酸 修饰电极 铅笔芯电极 不可逆双安培法 盐酸异丙嗪 

分 类 号：O657.15[理学—分析化学] R97[理学—化学]