苦参碱抑制人卵巢癌细胞株A21-2增殖与诱导凋亡的实验研究  

EXPERIMENTAL STUDY OF MATRINE ON INHIBITION OF PROLIFERATION AND INDUCTION OF APOPTOSIS IN HUMAN OVARY CANCER A21-2 CELLS

作　　者：李国慧[1] 颉玉欣[1] 郭晓华[1] 

机构地区：[1]河北医科大学第三医院实验中心,河北石家庄050051

出　　处：《河北医科大学学报》2008年第3期367-369,400,共4页Journal of Hebei Medical University

摘　　要：目的探讨苦参碱(matrine)抑制人卵巢癌细胞株A21-2增殖的作用及其分子机制。方法应用不同浓度的苦参碱作用于A21-2细胞,利用透射电镜观察细胞形态学变化。采用四甲基偶氮唑蓝法(tetrazolium bromide colorimetry,MTT)检测苦参碱对细胞的增殖抑制率;反转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测半胱氨酸蛋白酶-3(caspase-3)mRNA的表达。结果MTT实验表明苦参碱能抑制A21-2细胞的增殖,并呈良好的时间-剂量效应关系。经苦参碱作用A21-2细胞后,透射电镜下可见到细胞凋亡形态,RT-PCR显示caspase-3 mRNA的表达升高(P<0.01)。结论苦参碱能明显抑制A21-2细胞的增殖,诱导其凋亡,其机制可能与促进caspase-3表达有关。Objective To investigate effect of matrine on the proliferation and apoptosis of human ovary cancer A21-2 cells matrine and its molecular mechanisms. Methods A21-2 cells were treated with matrine at different concentration. Morphology changes of cell apoptosis were observed by transmission electron microscopy. The inhibition of proliferation was determined bY tetrazolium bromide colorimetry （MTT） method. The expression of caspase-3 mRNA was analysed by reverse transcription polymerase chain reaction. Results The growth of A21-2 cells were inhibited of in a time- and dose-dependent fashion by matrine. After matrine treatment,A21- 2 cells showed apoptosis morphological changes under transmission electron microscopy. And the expression of caspase-3 mRNA was up-regulated （ P 〈 0.01）. Conclusion Matrine can inhibit proliferation and induce apoptosis of A21-2 cells. It＇s molecular mechanism might be related to modulating the expression of caspase-3.

关 键 词：苦参碱 细胞凋亡 卵巢肿瘤 

分 类 号：R730.52[医药卫生—肿瘤]