重症肌无力增生型胸腺组织11KD差异蛋白带双向电泳图谱的建立  

Establishment of two-dimensional gel electrophoresis profiles of hyperplastic thymus with 11KD strip of myasthenia gravis patients

作　　者：李静孟[1] 高峰[2] 方华[2] 崔新征[1] 张建立[3] 刘素娜[2] 张清勇[1] 

机构地区：[1]郑州大学第二附属医院胸外科,郑州450014 [2]郑州大学河南省医药科学研究院,郑州450052 [3]河南中医学院,郑州450003

出　　处：《中国实用神经疾病杂志》2008年第5期30-31,共2页Chinese Journal of Practical Nervous Diseases

基　　金：国家自然科学基金资助项目(30570625)

摘　　要：目的建立分辨率高和重复性好的重症肌无力(MG)增生型胸腺组织11KD差异(异常)蛋白带的二维凝胶电泳图谱。方法首先采用SDS-PAGE电泳筛选出含有11KD差异(异常)蛋白条带的MG增生型胸腺组织,然后富集11KD蛋白条带。以17cm pH3-10固相pH梯度胶条(immobilized pH gradient,IPG)做第一向等电聚焦(IEF),12%SDS聚丙烯酰胺凝胶为第二向进行双向电泳(2-DE),PD Quest 7.1软件分析电泳图谱。结果11KD差异(异常)蛋白条带的出现率为72.2%;通过双向电泳,建立分辨率高和重复性好的重症肌无力(MG)增生型胸腺组织11KD差异蛋白带的双向凝胶电泳图谱,进一步分离11KD差异(异常)蛋白带,共得到(30±6)个蛋白点。结论通过蛋白质组技术快速建立重症肌无力11KD差异(异常)蛋白表达图谱,为进一步研究差异表达蛋白的功能提供实验基础。Objective To establish two-dimensional gel electrophoresis （2-DE） profiles of hyperplastie thymus with 11KD strip of myasthenia gravis patients. Methods The thymus tissue containing 11KD differential or abnormal strap was bolted with SDS-polyaerylamide gel eleetrophoresis. 11KD protein strips were collected and cleaned. The 2-DE system with the first dimension using isoeleetrofoeusing eleetrophoresis with 17cm pH3-10 IPG gel and the second dimension using 12 % SDS-poly-aerylamide gel eleetrophoresis were employed to obtain the 2-DE maps, and analyzed by PDQuest gel image software. Results 72.2% hyperplastie thymus tissues contained 11KD protein strip. The two-dimensional polyaerylamide gel eleetrophoresis profiles of 11KD proteins were successfully established by 2-DE and obtained （30±6） protein spots. Conclusion The 2-DE profiles of 11KD proteins were successfully established and can be an experimental basis for further research of myasthenia gravis.

关 键 词：重症肌无力 胸腺 蛋白质组学 双向电泳 

分 类 号：R746.1[医药卫生—神经病学与精神病学]