再灌注前后稀释血液对兔缺血再灌注心肌SOD和丙二醛的影响  

Effects of isovolumetric hemodilution before and after reperfusion on SOD and MDA of ischemic reperfused myocardium in rabbits

作　　者：姜文霞[1,2] 吕杰胜[1,2] 汤宁 鄢文海[1,2] 祁元明 黄幼田[1,2] 王柏生 

机构地区：[1]河南医科大学药学系 [2]河南医科大学病理生理学教研室

出　　处：《河南医科大学学报》1997年第4期9-11,共3页Journal of Henan Medical University

基　　金：河南省自然科学基金

摘　　要：观察应用右旋糖酐４０分别于再灌注前后稀释血液对兔缺血再灌注心肌ＳＯＤ活性和丙二醛（ＭＤＡ）含量的影响。心肌缺血４５ｍｉｎ，再灌注１８０ｍｉｎ，复制急性心肌缺血再灌注模型。３０只兔随机分为３组，每组１０只。其中Ⅰ组（对照组）、Ⅱ组于再灌注后２０ｍｉｎ稀释血液；Ⅲ组于再灌注前２０ｍｉｎ稀释血液。结果：与Ⅰ组相比，Ⅱ组ＳＯＤ活性无明显变化，ＭＤＡ含量明显降低（Ｐ＜００５）；而Ⅲ组ＳＯＤ活性明显升高及ＭＤＡ含量显著降低（Ｐ＜００５）。提示：再灌注前后稀释血液对缺血再灌注心肌均有保护作用；而且再灌注前稀释血液的效果明显优于再灌注后稀释。he purpose of the present study was to investigate the effects of hemodilution with Dextron 40 before and after reperfusion on contens of SOD and MDA of ischemic reperfused myocardium.Coronary artery was occluded for 45 min and then reperfused for 180 min in order to reproduce acute myocardial ischemiareperfusion models.A total of 30 rabbits were equally divided at random into three groups:Group Ⅰ (control),Group Ⅱ (hemodilution was performed 20min after reperfusion),and Group Ⅲ (hemodilution was performed 20min before reperfusion).Results:Compared with Group Ⅰ,SOD activities had no obvious difference,and MDA contents decreased significantly in Group Ⅱ(P<0.05);SOD activities and MDA contents significantly elevated and decreased in Group Ⅲ(P<0.05),respectively.The results indicated that hemodilution given before and after reperfusion could protect ischemic reperfused myocardium,and the effects of hemodilution administered before reperfusion were much better than after reperfusion.

关 键 词：缺血再灌注心肌 血液稀释 SOD 丙二醛 心肌缺血 

分 类 号：R363.27[医药卫生—病理学] R542.2[医药卫生—基础医学]