地塞米松预处理减轻大鼠再灌注性心律失常的实验研究  

Dexamethasone pretreatment attenuates reperfusion arrhythmia in rats

作　　者：庄梅[1] 方颖[1] 吴立荣[1] 雷大卫[2] 胡琴[3] 

机构地区：[1]贵阳医学院附属医院心内科,贵州贵阳550004 [2]贵阳医学院生化教研室,贵州贵阳550004 [3]山东大学齐鲁医院心内科,山东济南250012

出　　处：《中国病理生理杂志》2008年第5期862-866,共5页Chinese Journal of Pathophysiology

基　　金：贵州省优秀科技教育人才省长专项基金资助项目[No.黔省专合字(2007)118]

摘　　要：目的:探讨地塞米松预处理对大鼠再灌注性心律失常的作用及机制。方法:SD大鼠随机分成地塞米松组、对照组,分别予地塞米松和生理盐水预处理。预处理后构建缺血再灌注损伤动物模型,观察再灌注期间心律失常的发生;Western blotting法和免疫组化法观察心肌HSP72表达变化;测定心肌MDA、SOD、CAT、GSH-Px水平及心肌细胞膜Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性。结果:与对照组相比,地塞米松组室性心律失常的积分减少(P<0.01)、持续时间缩短(P<0.05);HSP72的表达增加(P<0.05);MDA降低(P<0.01),SOD、CAT、GSH-Px均升高(P<0.05);Na+-K+-ATP酶增加(P<0.01),Ca2+-Mg2+-ATP酶无明显变化(P>0.05)。结论:地塞米松预处理减少再灌注室性心律失常,其机制可能与其上调HSP72、Na+-K+-ATP酶、抗氧化酶的表达及抑制脂质过氧化反应有关。AIM： To investigate the effect of dexamethasone （DEX） preconditioning on reperfusion arrhythmia. METHODS： 46 Sprague-Dawley rats were divided randomly into DEX and control （CON） group, the rats were pretreated with DEX or sodium chloride before their hearts were separated for Langendorff perfusion and for ischemia/reperfusion. The reperfusion arrhythmias were observed dynamically after 60 min reperfusion following 30 min ischemia. The expression of HSP72 in myocardium was examined by Western blotting and immunohistochemistry at reperfusion 60 min. The levels of malondialdehyde （MDA） , superoxide dismutase （SOD） , catalase （CAT） , glutathione peroxidase （GSH-Px） and the activities of Na^＋ -K^＋ -ATP ase, Ca^2＋ - Mg^2＋ - ATPase on myocardial plasma membrane were detected. RESULTS： Compared with control group, the accumulated points and persistence time of ventricular arrhythmia were reduced significantly in DEX group （ P〈0.05 ） , the expression of HSP72 was significant upregulated （ P 〈 0. 05 ）, the level of MDA was reduced significantly, the activities of SOD, CAT, GSH-Px and Na^＋ -K^＋ -ATPase were significantly higher （P 〈 0. 05）. CONCLUSION： Dexamethasone pretreatment markedly reduces the reperfusion ventricular arrhythmias in rats, which may be attributed to upregulation of HSP72, SOD, CAT, GSH-Px , Na^＋ -K^＋ -ATPase and inhibition of lipid peroxidation.

关 键 词：地塞米松 心律失常 热休克蛋白质70 ATP酶 活性氧 

分 类 号：R363[医药卫生—病理学]