OX-LDL对巨噬细胞Toll样受体和炎症反应的影响以及GW1929的干预作用  

Effects of GW1929 on macrophage TLR expression and inflammation induced by ox-LDL

作　　者：李飞[1] 王景峰[1] 聂如琼[1] 罗年桑[1] 耿登峰[1] 袁沃亮[1] 谢双伦[1] 林永青[1] 赵文杰[2] 

机构地区：[1]中山大学医学院附属二院心血管科,广东广州510120 [2]中山大学医学院附属二院皮肤科,广东广州510120

出　　处：《中国病理生理杂志》2008年第5期909-914,共6页Chinese Journal of Pathophysiology

基　　金：国家“十五”科技攻关课题分课题资助项目(No.2004BA714B03)

摘　　要：目的:研究ox-LDL对巨噬细胞TLR2、TLR4表达和TNF-α、L-10、IL-12、NO以及MDA生成的影响并观察GW1929的干预作用。方法:ox-LDL(50mg/L;100mg/L)、GW1929(20μmol/L)作用于小鼠腹腔巨噬细胞24h后,测定培养液上清中MDA(TBA法)和NO(硝酸盐还原酶法)以及TNF-α、IL-10和IL-12(ELISA法)的浓度。采用流式细胞技术观察ox-LDL(50mg/L)作用于小鼠腹腔巨噬细胞6h、12h及24h后TLR2、TLR4的表达。结果:ox-LDL(50mg/L,100mg/L)组MDA、NO2-/NO3-、TNF-α以及IL-10的浓度均明显高于control与GW1929组;而ox-LDL(50mg/L,100mg/L)+GW1929组以上指标的浓度分别明显低于ox-LDL(50mg/L,100mg/L)组;各组培养液中均无检测到IL-12的生成。ox-LDL(6h、12h、24h)组以及ox-LDL+GW1929(6h、12h、24h)组TLR-2、TLR4的表达明显高于control与GW1929组。其中,ox-LDL+GW1929(6h、12h、24h)3组TLR-2的表达分别低于ox-LDL(6h、12h、24h)组;ox-LDL+GW1929(12h)组TLR-4的表达明显低于ox-LDL(12h)组。(P<0.05)。结论:ox-LDL上调了巨噬细胞TLR2及TLR4的表达,促进巨噬细胞活性氧、NO以及细胞因子TNFα、IL-10的生成,GW1929对抗了ox-LDL在以上过程中的作用。AIM ： To investigate the effects of ox-LDL on TLR2 and TLR4 expression and production of TNF- α, IL - 10, IL - 12, NO and MDA in macrophages and to observe intervention effect of GW1929 in above procedure. METHODS： The mouse peritoneal macrophages were pretreated with ox -LDL （50 mg/L, 100mg/L） and GW1929 （20μmol/L） respectively for 24 h. The concentrations of MDA, NO2-/NO3-, TNF-α, IL - 10 and IL - 12 in the culture fluid were detected. Flow cytometry was used to observe TLR2 and TLR4 expressions after the mouse peritoneal macrophages were pretreated with ox -LDL （50mg/L） and GW1929 （20μmol/L） respectively for 6 h, 12 h, and 24 h. RESULTS： The concentrations of MDA, NO2-/NO3-, TNF-α and IL - 10 in ox - LDL （50 mg/L, 100 mg/L） group were higher than those in control and GW1929 group obviously, but the concentrations of above index in ox - LDL （50mg/L, 100mg/L） ＋ GW1929 group were lower than those in ox - LDL （50mg/L, 100mg/L） group apparently. No IL - 12 in every group was detected. Expressions of TLR -2 in ox - LDL ＋ GW1929 （6 h, 12 h, 24 h） group were lower than those in ox - LDL （6 h, 12 h, 24 h） group respectively. TLR-4 expressions in ox-LDL ＋ GW1929 （ 12 h） were lower than those in ox-LDL （12 h） apparently. CONCLUSION： ox-LDL up-regulates TLR2 and TLR4 expressionsand promotes the production of ROX, NO, TNF-α and IL - 10 in macrophages. GW1929 is capable of inhibiting the above ox-LDL effects.

关 键 词：动脉硬化 炎症反应 细胞因子类 受体 Toll样 巨噬细胞 

分 类 号：R363[医药卫生—病理学]