Ad-14-3-3σ对不同辐射抗拒的鼻咽癌细胞微小RNA表达的影响  

Effect of Ad-14-3-3σ on microRNA expression in different radioresistant nasopharyngeal carcinoma cells

作　　者：王旭丹[1] 梁志慧[2] 杨惠玲[1] 唐兵[1] 赵睿颖 郭禹标[4] 郑诚[1] 郑芹[1] 

机构地区：[1]中山大学中山医学院病理生理学教研室,广东广州510080 [2]中山大学肿瘤防治中心,广东广州510060 [3]Department of Molecular and Cellular Oncology,University of Texas,MD Anderson Cancer Center,Houston,TX77030,USA [4]中山大学附属一院内科,广东广州510080

出　　处：《中国病理生理杂志》2008年第5期972-977,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30670627);广东自然科学基金资助项目(No.06021210);广东重大科技专项资助项目(No.30604);广东重点科技攻关资助项目(No.2004A30801006);广州市科技计划(国际合作)资助项目(No.2004J1-I0041)

摘　　要：目的:在明确Ad-14-3-3σ对不同辐射抗拒鼻咽癌(nasopharygycarcinoma,NPC)细胞CNE-1和CNE-2治疗作用的基础上,检测Ad-14-3-3σ对CNE-1和CNE-2细胞中差异表达的微小RNA(microR-NA,miRNA)的影响,探索CNE-1和CNE-2细胞中miRNA差异表达与NPC放射敏感性差异的关系。方法:用Ad-14-3-3σ转染CNE-1和CNE-2细胞;采用Paraflo microfluidic microRNA芯片检测,用激光扫描器收集杂交的图像,经LOWESS滤器规范信号后分析数据差异,根据Targetscan3.1数据库资料(http://www.targetscan.org)探索Ad-14-3-3σ对CNE-1和CNE-2中的miRNA差异表达的影响,预测其差异表达与NPC放射敏感性差异的关系。结果:Ad-14-3-3σ处理细胞以后,CNE-1与CNE-2相比较,有37个microRNA有明显差异,CNE-1中有17个上调,20个下调。其中倍数变化在3倍以上且2者检测量数量差异达到1000以上的有6个:hsa-miR-152、hsa-miR-205、hsa-miR-203、hsa-miR-7、hsa-miR-636和hsa-miR-100。结论:Ad-14-3-3σ能够改变微小RNA(microRNA,miRNA)在CNE-1和CNE-2中的表达模式,缩小CNE-1和CNE-2之间miRNA的表达差异,而这些miRNA可能跟肿瘤的发生和放疗敏感性有关。AIM： To discuss the effect of Ad-14-3-3σ to microRNA （miRNA） in different radioresistant nasopharyngeal carcinoma （NPC） cells, CNE - 1 and CNE - 2, and study the relationship between the discrepancy of miRNA and radiosensitivity of NPC. METHODS： Ad-14-3-3σ was transfected to CNE- 1 and CNE- 2 ceils, and then miRNAs were detected by Paraflo microfluidic microRNA chip. Hybridization images were collected using a laser scanner and the signals were normalized using a LOWESS filter. The effect of Ad-14-3-3σ to miRNAs and the relationship between the discrepancy of miRNA and radiosensitivity of NPC were studied according to Targetscan3.1 database （http：// www. targetsan. org） after analyzing data. RESULTS： After treated by Ad-14-3-3σ, comparing to CNE - 2 cells, there are 37 miRNAs changed remarkably, including 17 over - expression microRNAs and 20 under - expression microR- NAs in CNE - 1 ceils. 6 miRNAs that one detective value was more than 1000 and 3 folds than the other were hsa - miR - 152,hsa - miR -205 ,hsa - miR -203 ,hsa - miR -7 ,hsa - miR -636 and hsa - miR - 100. CONCLUSION： Ad-14-3-3σ can change the expression of miRNAs in different radioresistant nasopharyngeal carcinoma, and some miRNAs have relevance to carcinoma and radiosensitivity.

关 键 词：鼻咽肿瘤 MIRNA Ad-14-3-3σ 辐射耐受性 

分 类 号：R363[医药卫生—病理学]