缺血预处理诱导PC12细胞对缺血的耐受与缺氧诱导因子-1α的表达  

Effect of Ischemic Preconditioning on Ischemic Tolerance and Expression of HIF-1α in PC12 Cell Lines

作　　者：陈建[1] 朱俐[2] 潘永进[2] 肖峰[3] 姚茂元[3] 

机构地区：[1]南京医科大学附属淮安第一医院儿科,淮安市223300 [2]南通大学航海医学研究所 [3]南京医科大学附属淮安第一医院神经内科,淮安市223300

出　　处：《天津医药》2008年第5期328-330,共3页Tianjin Medical Journal

基　　金：江苏省高校自然科学基金资助项目(项目编号:04KJB310113)

摘　　要：目的:在PC12细胞水平上研究缺血预处理诱导的缺血耐受与缺氧诱导因子-1α(HIF-1α)的表达。方法:采用氧葡萄糖剥夺(OGD)的方法建立PC12细胞缺血模型。用MTT比色分析法观察随缺血时间的延长,细胞存活率的变化,观察短时间缺血预处理(IP)是否能在24h后诱导细胞耐受长时间的缺血。用WesternBlot法检测缺血预处理后HIF-1α的表达。结果:随缺血时间的延长,细胞存活率逐渐下降,缺血6.0h和9.0h存活率较低(P<0.01)。IP0.5h,IP1.0h和IP1.5h后间隔24h分别再经缺血6.0h和9.0h,细胞存活率均显著高于单纯缺血组,其中IP1.0h组高于IP0.5h组,IP1.5h组高于IP1.0h组。IP1.0h和IP1.5h组HIF-1α蛋白的表达均明显增加,IP1.5h组的蛋白表达量比IP1.0h组高(均P<0.01)。结论:IP0.5h和IP1.0h,IP1.0h和1.5h分别可以诱导PC12细胞对缺血6.0h和9.0h的耐受,其中IP1.0h对缺血6.0h的保护作用比IP0.5h强;IP1.5h对缺血9.0h的保护作用比IP1.0h强,与IP1.5h时HIF-1α蛋白表达高有关。Objective： To study the ischemic tolerance and the expression of hypoxia inducible factor -1α （HIF-1α） induced by ischemic preconditioning （IP） in PC12 cells. Methods： The ischemic model in PC12 cell line was developed with treatment of oxygen-glucose deprivation （OGD）. The time of ischemic tolerance of PC 12 ceils was examined with MTT assay. The expression of HIF-1α after IP was detected by Western Blot method. Results： With the increasing IP time,the cell viability gradually declined. The cell viability was significantly decreased at 6 h and 9 h （P 〈 0.01 ）. However,the cell viabilities were significantly increased in ischemia 6 h and 9 h after IP0.5 h, 1 h and 1.5 h compared with those of only ischemia 6 h and 9 h. In which,there was a higher cell viability in IP1 h than that of IP 0.5 h,and a higher cell viability in IP1.5 h than that of IP1 h. The expression of HIF-1α was significantly increased in IP1 h and IP1.5 h （P 〈 0.01 ）. The expression of HIF-1α was higher in IP1.5 h than that of IP1 h （P 〈 0.01 ）. Conclusion： The ischemic tolerance in PC12 ceils can be induced by IP0.5 h,IP1 h and IP1.5 h,in which there was a higher tolerance for ischemis 6 h induced by IP1 h than that of IP0.5 h, and a higher tolerance for 9 h induced by IP1.5 h than that of IP1 h. The effect may be related to the higher expression of HIF-1α at IP1.5 h.

关 键 词：缺血预处理 PC12细胞缺氧缺血 脑 缺氧诱导因子-1Α 

分 类 号：R735.34[医药卫生—肿瘤] R542.2[医药卫生—临床医学]