共刺激分子B7-H1在胃癌中表达上调  

Up-regulated expression of Co-stimulatory molecule B7-H1 in gastric carcinoma

作　　者：刘书漫[1] 李倩如[2] 张娓[1] 王一菱[1] 孟青[1] 张钦宪[1] 刘占举[3] 

机构地区：[1]郑州大学基础医学院组胚教研室,河南郑州450014 [2]郑州大学基础医学院微生物学与免疫学教研室,河南郑州450052 [3]郑州大学基础医学院郑州大学第二附属医院消化内科,河南郑州450014

出　　处：《基础医学与临床》2008年第5期450-454,共5页Basic and Clinical Medicine

基　　金：教育部新世纪优秀人才支持计划(NECT-05-0609);河南省重大科技攻关项目(0222031300)

摘　　要：目的了解胃癌患者胃黏膜组织中共刺激分子B7-H1的表达情况及其与肿瘤转移和预后的关系。方法应用流式细胞技术、免疫化学染色、免疫荧光染色与Western blot检测胃癌细胞系SGC-7901与新鲜切除的胃癌组织、癌旁组织及其远端正常胃黏膜组织中B7-H1的表达,并对相关数据进行相应的统计学分析,确定B7-H1表达水平与患者临床病理指标的关系。结果SGC-7901细胞中有B7-H1蛋白表达,主要分布在细胞膜,细胞质中少量;胃癌组织中B7-H1阳性表达率为(13/21)62%,癌旁组织中为(7/21)33%,而正常胃黏膜组织中没有B7-H1表达。统计分析显示,胃癌组织中B7-H1表达与肿瘤的浸润深度、周围淋巴结转移、远处转移及pTNM分期有关(P<0.05)。结论B7-H1分子可作为胃癌早期诊断与预后判断的新指标。Objective To study the expression of B7-H1 in gastric mucosa of to identify its relationship with neoplasm metastasis and prognosis. Methods patients with gastric carcinoma, and Expression of B7-H1 in gastric carcinoma cell line SGC-7901 and in freshly-resected gastric mucosa including gastric carcinoma, adjacent tumor tissue and distal normal gastric mucosa were examined by flow cytometric analysis, immunochemical staining, immunoflu- orescence staining and Western blot. The correlated data was analyzed statistically. And so the correlation among expression level of B7-H1 and the patients, clinicopathological parameters was established. Results B7-H1 expression was detected in SGC-7901 cell line. B7-H1 was found in cell membrane and little cytoplasm. The positive rate of B7-H1 expression in gastric carcinoma was （13/21）62% , and it was （7/21）33% in adjacent tumor tissue, Whereas B7-H1 was absent in distal normal gastric mucosa. Statistical analysis demonstrated a positive correlations of B7-H1 expression in gastric carcinoma with the depth of carcinoma infiltration, lymph node metastasis and pTNM stage （P 〈 0. 05 ）. Conclusion B7-H1 molecule may be used as a new marker to diagnose gastric carcinoma on early stage and to predict prognosis.

关 键 词：胃癌 B7-H1 转移 预后 

分 类 号：R735.2[医药卫生—肿瘤]