olomoucine对星形胶质细胞增殖的影响及机制研究  

The Effect of Olomoucine on Cultured Astrocyte Proliferation and its Mechanism Research

作　　者：朱舟[1] 徐逸[1] 潘邓记[1] 余勇飞[1] 陈华先[1] 王伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经科,武汉430030

出　　处：《神经损伤与功能重建》2008年第2期72-76,共5页Neural Injury and Functional Reconstruction

摘　　要：目的:观察细胞周期抑制剂olomoucine对培养星形胶质细胞(AS)机械损伤后增殖的影响,并探讨其作用机制。方法:建立体外培养大鼠纯化的AS物理损伤模型(划痕损伤模型),随机分为对照组、划痕组和干预组,划痕组和干预组均继续培养,干预组给予olomoucine干预。利用免疫荧光细胞化学方法观察损伤后BrdU表达;利用Westernblot印迹分析损伤后PCNA、p27的表达。结果:划痕损伤刺激12h开始出现损伤边缘区AS胞体肥大、数目增加,BrdU阳性细胞比率和PCNA表达水平较对照组增高(P<0.05),p27表达水平较对照组下降(P<0.05);干预组的BrdU阳性细胞比率和PCNA表达水平较划痕组明显减少(P<0.05),p27表达上调(P<0.05)。结论:损伤刺激可促使AS发生反应性胶质增生,CDK选择性细胞周期抑制剂olomoucine可以通过调控细胞周期抑制其反应性增生。Objective：To observe the effect of CDK inhibitor-olmoucine on cultured astrocyte proliferation after mechanical injury and to explore the mechanism. Methods： The model of mechanical injury on cultured purified astrocytes was established. The cultured cells were randomly divided into 3 equal groups： control group, scratch group （scratch ＋ vehicle） , and olomoucine group （scratch ＋ olomoucine）. Immunofluorescence （IF） staining was used to detect the expression of glial fibriliary acidic protein （GFAP） and BrdU. Western bolt was used to detect the expression of PCNA and p27. Results：The ＂scratch-wound injury＂ can induce AS proliferation. From 12 hours after injury, AS around the injury area appeared hypertrophic and proliferative. The percent of the BrdU-positive cells and the expression of PCNA increased（P〈0.05） ,while the expression of p27 decreased（P〈0.05）. In the olomoucine group,the percent of the BrdU-positive cells and the expression of PCNA were be inhibited significantly by olomoucine（P〈0.05）. Conclusion： As a response of injury, AS entered cell cycle and appeared reactive gliosis. The cell cycle inhibitor,olomoucine can inhibit injury-induced reactive gliosis by regulating cell cycle.

关 键 词：星形胶质细胞 细胞周期 OLOMOUCINE 增殖 活化 

分 类 号：R741[医药卫生—神经病学与精神病学] R322.81[医药卫生—临床医学]