自体骨髓间充质干细胞移植密度与Beagle犬牙Ⅱ度根分叉病变组织修复的关系(英文)  

Different densities of autologous bone marrow mesenchymal stem cell transplantation for repairing Beagle canine teeth class Ⅱ furcation defects

作　　者：詹暶[1] 闫福华[1] 肖毅 

机构地区：[1]福建医科大学附属口腔医院牙周科,福建省福州市350002 [2]Institute of Health and Biomedical Innovation Queensland University of Technology, Australia

出　　处：《中国组织工程研究与临床康复》2008年第16期3193-3197,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：福建省教育厅科技项目基金(JA05267);福建医科大学第六批青年骨干教师基金(闽医大[2006]124号)~~

摘　　要：背景:目前临床上II度根分叉病变区牙周组织的再生仍是一项难题。目的:比较不同密度自体骨髓间充质干细胞修复II度牙根分叉病变区牙周组织缺损的能力。设计:随机对照观察实验。单位:福建医科大学附属口腔医院实验室和福州总医院动物实验科。材料:实验于2005-07/2006-09在解放军南京军区福州总医院动物实验科和福建医科大学附属口腔医院实验室完成。6只1岁半雄性Beagle犬由解放军南京军区福州总医院动物实验科提供,实验过程中对动物处置符合动物伦理学标准。实验中选用Bio-Gide胶原膜和BME-10X胶原膜。方法:在犬的下颌第2,3前磨牙和第1磨牙颊侧根分叉处制备慢性II度牙根分叉病变模型。从6只18月龄的Beagle犬抽取自体骨髓间充质干细胞。应用5×108L-1,5×109L-1,5×1010L-1的骨髓间充质干细胞复合BME-10X胶原膜移植治疗牙根分叉病变,表面覆盖Bi0-Gide胶原膜,单纯Bi0-Gide胶原膜组为对照组。采用OLYPUS IX71显微照相系统和OLYSIA BioAutoCell软件计算新生牙骨质长度的百分比和新生牙槽骨面积的百分比。主要观察指标:苏木精-伊红染色观察并测量牙周组织再生(新生牙骨质长度和新生牙槽骨面积的百分比)情况。结果:对照组的新生牙骨质长度和新生牙槽骨面积的百分比分别为:(51.5±5.6)%和(27.1±7.7)%;5×108L-1骨髓间充质干细胞组为:(84.8±8.9)%和(30.6±7.7)%;5×109L-1骨髓间充质干细胞组为:(91.8±5.2)%和(68.3±11.4)%;5×1010L-1骨髓间充质干细胞组为:(88.8±7.2)%和(78.5±12.7)%。细胞组的新生牙骨质量与对照组相比,差异有显著性意义(P<0.01),但各细胞组间相互比较差异无显著性意义;5×109L-1组和5×1010L-1组的新生牙槽骨量与5×108L-1组和对照组相比差异有显著性(P<0.05),但前两组间和后两组间比较则差异无显著性意义。结论:骨髓间充质干细胞移植能促进牙根分叉病变区的牙周组织再生,其作�BACKGROUND： Regeneration of type Ⅱ furcation defects of periodontal tissues is still a great clinical challenge. OBJECTIVE： To compare different densities of autologous bone marrow mesenchymal stem cells （auto-BMSCs） for repairing canine experimental class Ⅱ furcation defects of periodontal tissues. DESIGN： A randomized controlled trial. SETTING： Laboratory in Stomatological Hospital Affiliated to Fujian Medical University and Department of Animal Experiment in Fuzhou General Hospital. MATERIALS： Experiments were performed at the Laboratory in Stomatological Hospital Affiliated to Fujian Medical University and Department of Animal Experiment in Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from July 2005 to September 2006. Six 18-month Beagle dogs were provided by Department of Animal Experiment in Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLAt. Animal intervention met animal ethical standards. Bio-Gide collagen membrane and BME-10X collagen membrane were used in the study. METHODS： Class Ⅱ furcation defects were induced surgically on the buccal side of canine mandibular second and third premolar （P2, P3） and first molar （M1）. The ex vivo expanded auto-BMSCs from six 18-month Beagle dogs were seeded in BME-10X collagen membranes at cell density of 5 × 10^8 ·L^-1, 5 × 10^9 ·L^-1, 5× 10^10 ·L^-1, and delivered into experimental class li furcation defects, underneath a Bio-Gide membrane. Bio-Gide membrane alone was used as a control. The percentage of new cementum length and percentage of new alveolar bone area were measured on OLYPUS IX 71 inverted research microscope and OLYSIA BioAutoCell software in a computer. MAIN OUTCOME MEASURES： Each specimen was stained with hematoxylin and eosin. The lengths of new cementum and the area of new alveolar bone were calculated. RESULTS： The percentage of newly formed cementum length and the percentage of newly formed alveolar bone area were （51.5 ± 5.6）% and （27.1 ± 7.7

关 键 词：牙周组织再生 骨髓间充质干细胞 细胞密度 

分 类 号：R394.2[医药卫生—医学遗传学]