硝酸铕对蚕豆根尖细胞遗传毒性的研究  

Genetic Toxicity of Europium Nitrate to Vicia faba Root Tip Cells

作　　者：孙玲[1] 张惠芳[1] 杨瑞卿[1] 

机构地区：[1]徐州工程学院环境工程学院,江苏徐州221008

出　　处：《环境与健康杂志》2008年第5期423-425,共3页Journal of Environment and Health

基　　金：徐州工程学院青年教师基金资助项目(XKY2007321)

摘　　要：目的探讨硝酸铕对蚕豆根尖细胞的遗传毒性。方法将蚕豆初生根浸入铕系列溶液(64、32、16、8、4、2、1μg/ml)中,25℃恒温培养6h,之后双蒸水中修复培养24h。切取根尖,固定、染色、镜检,统计微核率、有丝分裂指数和染色体畸变率。结果在1～32μg/ml浓度范围内,微核率随硝酸铕处理剂量的增加而升高,呈剂量-效应关系(r=0.949)。在2～64μg/ml浓度范围内,有丝分裂指数随硝酸铕处理剂量的增加而下降,呈剂量-效应关系(r=-0.852)。在1～64μg/ml浓度范围内,染色体断片和总畸变率随Eu(NO3)3处理剂量的升高而上升,且具有明显的剂量-效应关系(r剂量-断片=0.915,r剂量-总畸变率=0.872)。结论硝酸铕对蚕豆根尖细胞具有一定的遗传毒性,染色体断片可能是形成微核的原因之一。Objective To evaluate the genotoxicity of europium nitrate to the Viciafaba L root-tip cells. Methods The primary roots of ViciafabaL were cultured for 6 hours in the solution of Eu series（64, 32, 16, 8, 4, 2, 1 μg/ml） at 25℃.And then the roots were repair-cultured in double distilled water for 24 h. The root tips of Viciafaba L were dissected and fixed, stained and the micronucleus rate, mitotic index and the rate of chromosomal aberrations were determined. Results From 1 μg/ml to 32 μg/ml, the rate of micronucleus increased with the increasing of the europium nitrate dosage （D）, presented a dosage-effect relationship （r=0.949）. From 2 μg/ml to 64 μg/ml, mitotic index decreased with the increasing of the europium nitrate dosage, presented a dosage-effect relationship（r=-0.852）. From 1 μg/ml to 64 μg/ml, chromosome fragments （CF） and frequency of chromosomal aberrations（FCA） increased with the increasing of the europium nitrate dosage with a dosage-effect relationship significantly（rD-CF= 0.915, rD-FCA-0.872）. Conclusion Europium nitrate has the certain genetic toxicity to the Viciafaba L root-tip cells. The chromosome fragments may be one of the reasons to produce micronucleus.

关 键 词：稀土 铕 蚕豆 微核试验 有丝分裂指数 染色体畸变 

分 类 号：R994.6[医药卫生—毒理学]