乌司他丁对重症急性胰腺炎大鼠脾淋巴细胞功能的影响  

Effects of Ulinastatin on the function of splenic lymphocytes from rats with severe acute pancreatitis

作　　者：吴双虎[1] 马涛[1] 邱轶伟[1] 邵宏伟[1] 薛承锐[1] 胡文全[1] 

机构地区：[1]天津医科大学总医院外科,天津300052

出　　处：《中华急诊医学杂志》2008年第5期496-498,共3页Chinese Journal of Emergency Medicine

基　　金：天津市自然科学基金资助（05YFTJMJC14700）

摘　　要：目的观察乌司他丁对重症急性胰腺炎（Severe acute pancreatitis，SAP）大鼠脾淋巴细胞功能的影响。方法清洁级Wistar大鼠28只随机分为对照组、假手术组、SAP组和乌司他丁组。对照组不施行手术，假手术组不给予胆胰管内注入脱氧胆酸钠，余同SAP模型组。乌司他丁组在制作SAP模型后30min经尾静脉注射乌司他丁5万U／kg，其他3组均注入等量生理盐水。术后24h处死大鼠，分离培养脾淋巴细胞，MTT法检测脾淋巴细胞增殖活性，ELISA法检测脾淋巴细胞培养上清中Th1细胞因子IL-2、IFN-γ和Th2细胞因子IL-10水平。计量资料以均数±标准差（-↑x±s）表示，采用SPSS10．0统计软件包进行数据处理。组间比较采用Student’t检验。以P〈0．05为差异具有统计学意义。结果与假手术组相比，SAP组脾淋巴细胞增殖活性明显下降，Th1和Th2细胞因子产生均明显减少。与SAP组相比，乌司他丁组脾淋巴细胞增殖活性明显升高，Th1和Th2细胞因子生成也明显增加。结论SAP脾淋巴细胞增殖障碍，且Th1／Th2平衡失调，应用乌司他丁有助于改善脾淋巴细胞功能异常，减轻SAP特异性免疫功能障碍。Objective To investigate the effects of Ulinastatin （UTI） on the function of splenic lymphocytes from rats with severe acute pancreatitis （SAP）. Method Twenty-eight Wister rats （ clean grade） were randomly divided into control, sham operation, SAP, and ulinastatin group. No operation was performed in control group. And rats with sham-operation received laparotomy and catheterization into choledocho-pancreatic duct without injection of sodium deoxycholic. Rats in ulinastatin group received ulinastatin injection （50 000 U/kg） via tail vein 30 minutes after pancreatitis induced with DCA injected into pancreatic duct. Rats of other groups were given equal volume of saline. At 24 hours after operation, all animals were killed by neck dislocation, and splenocytes were isolated and cultured in RPMI 1640 medium containing 10% fetal calf serum. Proliferation of splenocytes was determined with MTT cellular preliferation assay. Levels of Th1 cytokines （IL-2, IFN-γ） and Th2 cytokine （IL-10） in supematants of splenocytes were measured by ELISA. Quantitative data were expressed as mean ± SE. Statistical analyses were performed by Student＇s t test with SPSS software （version 10.0 for Windows）. A P value less than 0.05 was considered statistically significant. Results The concentration of IL-2, IL-10 and IFN-γ and preliferative activity of splenocytes in SAP group were significantly lower than that in sham operation group. In contrast, the preliferative as well as the cytokine-releasing capacities of the splenocytes from rats treated with UTI were significantly increased compared with those from rats with SAP. Conclusions The deficiencies in prmliferation and cytokine release in response to antigen stimulation inaplys an anergic state of splenocytes during SAP. Treatment with UTI contributed to the recovery of the immune function by improving preliferative responses and cytokine release of splenocytes.

关 键 词：乌司他丁 重症急性胰腺炎 脾淋巴细胞 TH1 TH2 

分 类 号：R57[医药卫生—消化系统]