医化废水驯化过程中细菌遗传多样性的RAPD分析  

Genetic Diversity of Bacteria during the Domestication of Medical and Chemical Wastewater Using RAPD Techniques

作　　者：金莹[1] 李钧敏[1] 赵晓兰[1] 郑婷婷[1] 陈旭林[1] 余彬彬[1] 

机构地区：[1]台州学院生态研究所,浙江临海317000

出　　处：《环境科学研究》2008年第3期151-154,共4页Research of Environmental Sciences

基　　金：浙江省科技厅面上科研项目(2007C23052)

摘　　要：利用RAPD分子标记技术分析了医化废水驯化过程中细菌的遗传多样性.结果表明:9种引物在5种浓度的废水驯化细菌中共扩增出134条带,其中88条表现为多态性,多态性位点百分率为65.7%.根据Jacaard相异系数转换的距离矩阵,富集细菌与10%医化废水驯化细菌及20%医化废水驯化细菌之间的遗传距离最大,为0.750 0;而20%医化废水驯化细菌与40%医化废水驯化细菌之间遗传距离最小,为0.545 5.UPGMA法聚类结果表明,富集细菌与5%医化废水驯化细菌聚在一起组成一组;20%医化废水驯化细菌与40%医化废水驯化细菌先聚在一起,再与10%医化废水驯化细菌聚在一起组成另一组.Using RAPD techniques, the genetic diversity of bacteria during the domestication of medical and chemical wastewater was analyzed. 134 bands were amplified among five kinds of bacteria which were domesticated by medical and chemical wastewater with different content, among which 88 bands were polymorphic and the percentage of polymorphic loci was 65.7%. According to the Jacaard dissimilarity coeglcient, the genetic distance between bacteria domesticated by 0% （volume fraction） wastewater and bacteria domesticated by 5 % （volume fraction） wastewater or 20% （volume fraction） wastewater were the largest （0.750 0）, while that between the bacteria domesticated by 20% （volume fraction） wastewater and those domesticated by 40% （volume fraction） wastewater were the lowest （0.545 5）. The clustering results using UPGMA showed that the enriched bacteria and the bacteria domesticated by 10% （volume fraction） wastewater were cluster into one group, while bacteria domesticated by 20% （volume fraction） wastewater and that domesticated by 40% （volume fraction） wastewater were clustered first and then cluster with that domesticated by 10% （volume fraction） wastewater into another group.

关 键 词：医化废水 驯化 细菌 遗传多样性 RAPD 

分 类 号：X172[环境科学与工程—环境科学]