核干因子在前列腺癌中的表达及其临床意义  

Expression of Nucleostemin in Prostate Cancer Tissues and Its Clinical Significance

作　　者：刘冉录[1] 徐勇[1] 张志宏[1] 王萌[1] 孙建涛[1] 齐士勇[1] 张玥[1] 李胜芝[1] 

机构地区：[1]天津医科大学第二医院泌尿外科/天津市泌尿外科研究所,天津300211

出　　处：《中华男科学杂志》2008年第5期418-422,共5页National Journal of Andrology

基　　金：天津市教育委员会(20050212);天津市科学技术委员会(033111311)项目

摘　　要：目的:探讨核干因子(NS)基因在前列腺癌组织中的表达及其临床意义。方法:利用RT-PCR及免疫组化方法对前列腺癌、良性前列腺增生(BPH)及高级别前列腺上皮内瘤(HGPIN)组织中NS基因的表达进行检测,并探讨NS蛋白表达水平与前列腺癌患者临床指标的关系。结果:前列腺癌中NSmRNA表达水平显著高于BPH中的表达水平,NS蛋白在前列腺癌中的强阳性、阳性及弱阳性表达率分别为48.8%、36.6%及12.2%,在BPH中分别为4.0%、32.0%及56.0%,在HGPIN中分别为5.0%、25.0%及60.0%,在前列腺癌中的表达水平显著高于BPH及HGPIN中的水平,具有统计学意义(P<0.05)。NS基因表达水平与前列腺癌的分化程度呈负相关,细胞分化程度越差,NS基因表达水平越高。结论:前列腺癌中高表达NS基因,NS基因在前列腺癌的不良分化和恶性增殖中可能起着重要作用。Objective： To explore the expression of the nucleostemin （NS） gene in prostate cancer （PCa） tissues and its clinical significance. Methods： We detected the NS expression in PCa, benign prostatic hyperplasia （BPH） and high grade prostatic intraepithelial neoplasia （HGPIN） tissues by RT-PCR and immunohistochemistry, and analyzed the correlation between the expression of the NS protein and the clinical variables of PCa. Results ： The NS mRNA level was markedly higher in the PCa than in the BPH tissues. The rates of strongly positive, positive and weakly positive expressions of the NS protein were 48.8%, 36.6% and 12.2% in PCa, 4.0%, 32.0% and 56.0% in BPH, and 5.0%, 25.0% and 60.0% in HGPIN, respectively. The expression level of the NS protein was significantly higher in PCa than in BPH and HGPIN （ P 〈 0.05 ）. The expression of the NS gene was negatively correlated with the degree of cell differentiation in the PCa tissues, the worse the differentiation, the higher the NS expression level. Conclusion ： The NS gene is highly expressed in PCa tissues and may have an important role in the adverse differentiation and malignant proliferation of prostate cancer.

关 键 词：核干因子 前列腺癌 细胞分化 增殖 

分 类 号：R737.25[医药卫生—肿瘤]