卒中易感型自发性高血压大鼠脑组织中基质金属蛋白酶及组织型基质金属蛋白酶抑制剂表达与活性变化  

Expression and activity of cerebral matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in stroke-prone spontaneously hypertensive rats

作　　者：涂悦[1] 曾山[2] 姚旻[2] 朱勇[2] 周欣[2] 李玉明[2] 

机构地区：[1]中国人民武装警察部队医学院附属医院脑系科中心,天津300162 [2]中国人民武装警察部队医学院附属医院武警部队心血管病研究所,天津300162

出　　处：《中风与神经疾病杂志》2008年第2期161-164,共4页Journal of Apoplexy and Nervous Diseases

基　　金：武警总部科研基金项目(WY2005-5)

摘　　要：目的研究卒中易感型自发性高血压大鼠(SHR-SPs)脑组织中基质金属蛋白酶(MMPs)以及组织型基质金属蛋白酶抑制剂(TIMPs)表达与活性的变化。方法观察期间每两周测定各组大鼠的收缩压。6个月后,采用Kaplan-Meier生存分析比较两组大鼠的发病率和病死率;以蛋白质印迹及明胶酶谱法分析两组中存活大鼠脑组织MMP-2和MMP-9蛋白水平和活性,并采用蛋白质印迹及逆向明胶酶谱法分析存活大鼠脑组织TIMP-1和TIMP-2蛋白表达和活性。结果SHR-SPs组收缩压明显高于对照组(P<0.01);观察期间SHR-SPs组80%大鼠发生脑卒中,50%出现卒中性死亡;SHR-SPs中存活大鼠脑组织MMP-2蛋白表达及活性较对照组增高(P<0.05);存活SHR-SPs脑组织TIMP-2蛋白表达和活性明显高于对照组(P<0.05),TIMP-1无明显变化。结论SHR-SPs脑组织中MMP-2的表达及活性均增高,同时TIMP-2的含量及活性呈现代偿性增高。Objective To investigate expression and activity of cerebral matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in stroke-prone spontaneously hypertensive rats （SHR-SPs）. Methods Systolic blood pressure （SBP） was measured in every two weeks. Incidences of stroke and mortality rate were compared by Kaplan-Meier survival analysis 6 months later. Western bolt, gelatin-zymography, and reverse-zymography were performed to detect protein expression, activity of gelatinases and their inhibitors in brain homogenates, respectively. Results SBP was greatly elevated in SHR-SPs at the end of follow-up（P 〈0.01 ）. 80% of SHR-SPs were presented with stroke symptoms and mortality rate was 50%. The expression and activity of MMP-2 were increased compared with those of controls. The expression and activity of TIMP-2 were increased compared with those of controls, and TIMP-1 remained unchanged in all groups. Conclusion The results of present work showes that both the expression and the activity of MMP-2 in the brain of SHR-SP were significantly higher than control, and the expression and the activity of TIMP-2 are reciprocally elevated.

关 键 词：高血压 基质金属蛋白酶 组织型基质金属蛋白酶抑制剂 脑卒中 

分 类 号：R743.3[医药卫生—神经病学与精神病学]