聚乙二醇模拟干旱对拟南芥芥子油苷的影响  

Effects of drought stress simulated by PEG on glucosinolates in Arabidopsis thaliana

作　　者：任欢[1] 戴绍军[1] 阎秀峰[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《黑龙江大学自然科学学报》2008年第2期241-245,共5页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金海外青年学者合作研究基金项目(30528013);国家自然科学基金资助项目(30670325);新世纪优秀人才支持计划(NCET-05-0328)

摘　　要：以PEG-6000处理模拟干旱胁迫,观察了营养生长时期拟南芥幼苗对干旱胁迫的生理响应及莲座叶中芥子油苷组成和含量的变化。结果发现,干旱胁迫下拟南芥莲座叶的叶片含水量下降、细胞膜透性(叶片相对电导率)和丙二醛含量增加,受害程度随处理时间而增强。干旱胁迫下莲座叶芥子油苷的组分未发生变化,总量先期高于对照、处理5d时迅速下降且与对照差异显著。同族芥子油苷对干旱胁迫的响应较为相似,脂肪族芥子油苷对干旱胁迫的响应比较明显且与总芥子油苷含量的变化趋势基本一致,而吲哚族芥子油苷对干旱胁迫则不敏感。Drought stress was simulated by PEG - 6000 （0. 06 g · mL^- 1 ） for 5 days. Seedlings of Arabidopsis thaliana （Columbia ecotype） were exposed to drought stress during vegetative stage. The change of leaf water content, relative conductance ratio, MDA content and glucosinolates content in rosette leaves of Arabidopsis thaliana during drought stress were studied. The results showed that the leaf water content of rosette leaves was obviously decreased, the relative conductance ratio and MDA content were increased during drought stress. At the same time, drought stress treatment during vegetative growth stage made glucosinolates content in rosette leaves of Arabidopsis thaliana lower than reference, but the composition of glucosinolates was not changed. The content of aliphatic glucosinolates were obviously decreased, but the indole glucosinolates were not significantly changed. Aliphatic glu- cosinolates were more sensitive to drought stress than indole glucosinolates.

关 键 词：拟南芥 芥子油苷 干旱 

分 类 号：Q948.11[生物学—植物学]