应用响应曲面试验设计法研究克百威气相色谱测定中的色谱行为  

Application of Response Surface Experimental Designing to the Study on the Chromatographic Behavior of Carbofuran in Its GC Determination

作　　者：丁振华[1] 胡艳云[2] 盛旋[2] 郑平[2] 

机构地区：[1]马鞍山市产品质量监督检验所,马鞍山243000 [2]安徽出入境检验检疫局,合肥230061

出　　处：《理化检验（化学分册）》2008年第5期395-398,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：应用响应曲面试验设计法研究了气相色谱不同的进样方式,包括常规分流不分流进样以及柱头进样对克百威色谱行为的影响。通过构建模型,对试验的影响因子包括进样口温度、初始柱温和载气流速对克百威色谱行为的相对重要性进行了评价。并利用面积归一化方法,对进样口温度和初始柱温对克百威分解的影响进行了定量评价和探讨。结果表明:在常规进样方式中,进样口温度和初始柱温是影响克百威分解的主要因素。通过色谱条件的优化,解决了常规进样不能解决的克百威的热分解问题。Effect of different modes of sample introduction, including the conventional separate-streaming or non-separate-streaming sample introduction and the head-column sample introduction, on the chromatographic behavior of carbofuran in the GC determination was studied by the method of response surface experimental designing. By studying with the mathematical model established, relative significance of the influential factors, i. e. temperature at the sample inlet, initial column temperature and flow-rate of carrier gas, as related to the thermodecomposition of carbofuran was evaluated. As judged by area normalization, the effects of sample inlet temperature and initial column temperature were evaluated quantitatively, and it was concluded that these 2 factors were the main cause for the thermodecomposition of carbofuran, which was proved to be unavoidable in the conventional mode of sample introduction, even under the optimized working conditions.

关 键 词：气相色谱-质谱 响应曲面 克百威 柱头进样 

分 类 号：O657.7[理学—分析化学]