溴酚蓝离子对萃取分光光度法测定长春花中总生物碱  

Ion-Pair Extraction-Spectrophotometric Determination of Total Alkaloids in Catharanthus Roseus with Bromophenol Blue as Color Reagent

作　　者：杨磊[1] 刘玉[1] 张琳[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《理化检验（化学分册）》2008年第5期427-429,432,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家科技支撑计划项目(2006BAD18B04);国家林业局重点科学技术研究项目(2006-58)

摘　　要：以文多灵为对照品，溴酚蓝为显色剂，在pH3．0缓冲溶液条件下于30℃显色5min，用三氯甲烷萃取，于413nm波长处采用离子对萃取分光光度法测定长春花中总生物碱的含量。结果表明：文多灵在0．04～0．28g·L^-1浓度范围内与吸光度呈线性关系，回归方程为y=0．1824X－0．03，相关系数为0．9993，文多灵的平均回收率为99．49％，总生物碱样品在10～50min内测定稳定。经测定长春花的根、茎、叶、花、种子等不同部位中总生物碱含量分别为2．230％，0．411％，1．098％，0．503％和0．013％。In a citric acid-phosphate buffer of pH 3.0, an ion-pair complex was formed between alkaloids in the sample and the color reagent bromophenol blue reacted at 30 ℃ for 5 min. The complex was extracted with CHCl3 in which the absorbance was measured at the wavelength of 413 nrn. Pure vindoline （purity： 〉99%） was used as reference standard in the preparation of calibration curve. Linear relationship between the absorbance and concentration of vindoline was obtained in the range of 0.04-0. 28 g·L^-1 , corresponding to the linear regression equation Y=0. 182 4 X-0. 03 （r=0. 999 3）. On the base of a known sample, different amounts of standard solution of vindoline were added separately to 8 portions of the sample solution to test for recovery of the method, the average value of recovery obtained was 99.49%. Total alkaloids in different parts of Catharanthus roseus were determined by the proposed method, giving results of 2. 230% （in roots）, 0. 411% （in stems）, 1. 098% （in leaves）, 0. 503% （in flowers） and 0. 013% （in seeds）.

关 键 词：离子对萃取 分光光度法 长春花 总生物碱 溴酚蓝 

分 类 号：O657.31[理学—分析化学]