枯草杆菌脂肪水解酶LipA和LipB  

Lipolytic Enzymes LipA and LipB from Bacillus subtilis

作　　者：赵博[1] 高仁钧[1] 廉虹[1] 曹淑桂[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130023

出　　处：《中国生物化学与分子生物学报》2008年第5期419-425,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.30400081;20432010;20672045)~~

摘　　要：源自枯草杆菌的分泌型脂肪水解酶LipA及LipB已经被克隆、表达并表征.它们的分子结构特点、催化机理也已经被深入研究.枯草杆菌脂肪水解酶因为具有较好的食品工业及化学工业等方面的应用潜力,已经吸引了越来越多的关注.通过定向进化及高通量筛选的方法对酶分子进行改造,提高其热稳定性及立体选择性是近年的研究热点.结合国外及本研究组的工作,本文对LipA和LipB的生化性质、结构特点以及采用基因工程突变的方法进行分子改造等方面的研究进展做一简要综述.另外,对其中一些研究论文做了简要的评价,并提出对未来工作的展望.Lipolytic enzymes LipA and LipB were cloned from Bacillus subtilis, and can be obtained in secretory forms from the culture medium. The crystal structures and catalytic characterizations of both enzymes had been extensively studied. B. subtilis lipolytic enzymes are attractive for their potential applications in food and chemistry industries. Directed evolution combined with in vitro high-throughput screening to improve the thermostability and enantioselectivity became the research focus of these enzymes. This review will discuss the biochemical properties, three-dimensional structures and molecular modification of LipA and LipB enzymes and their mutants, Additionally, this article will also provide a literature summarization and the prospects of future studies.

关 键 词：枯草杆菌 脂肪水解酶 酶结构 定向进化 

分 类 号：Q55[生物学—生物化学]