“益坤宁”对自然老化大鼠性激素的调节及卵巢修复的研究  

"Yi Kun Ning"and Its Effects on Regulating Aging Rats' Sex Hormone and on Ovary Repair

作　　者：张广美[1] 齐群艳[2] 张旸旸[2] 廖希慧[2] 刘国艺[3] 崔清志[3] 肖晓辉[3] 

机构地区：[1]哈尔滨医科大学第一临床医学院,哈尔滨150010 [2]黑龙江中医药大学,哈尔滨150040 [3]哈尔滨医科大学生理教研室,哈尔滨150001

出　　处：《世界科学技术-中医药现代化》2008年第2期69-73,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金课题(30670484)中药益坤宁治疗围绝经期综合征药靶识别及作用机理的研究;黑龙江省自然科学基金(D2005-15)中药益坤宁治疗围绝经期综合征药靶识别及作用机理的研究

摘　　要：目的:研究中药益坤宁对围绝经期大鼠卵巢功能和结构的影响。方法:选用11～13月龄雌性围绝经期wistar大鼠32只。随机将其分为模型组、益坤宁组、西药对照组。另选青年大鼠10只,月龄4个月,作为青年对照组,采用血清性激素分泌水平、血管内皮生长因子(VEGF)、颗粒细胞调亡及细胞形态等指标来观察益坤宁对卵巢结构和功能的影响。结果:益坤宁组与模型组比较,各项指标差异均有显著性或极显著性(P<0.05或P<0.01)。结论:研究证实益坤宁可升高围绝经期大鼠血清E2、P水平,降低血清FSH、LH水平;对围绝经期大鼠卵巢VEGF有促进作用;对卵巢颗粒细胞凋亡有抑制作用,提示益坤宁可以阻止卵泡闭锁,从而达到预防和延缓卵巢衰老的作用机制。We made an experiment to observe the effects of Yi Kun Ning on the functionalities and structures of perimenopause （PM） rats＇ ovary. In the experiment, thirty-two female wistar rats aged between 11 and 13 months （PM） were divided into a model group, a Yi Kun Ning group, and a western drug group. 10 other female rats aged 4 months were selected as a young control group. Sex hormone level, vascular endothelial growth factor （VEGF）, granulosa cell apoptosis, cell morphology, and other indicators were measured to observe the effects of Yi Kun Ning on the functionalities and structures of rats＇ ovary. Experimental results show that there is a significant or very significant （P 〈0.05, P 〈0.01） difference between the Yi Kun Ning group and the model group. The experiment confirms that Chinese medicine Yi Kun Ning is able to increase serum E2 and P levels in PM rats, with a reduced FSH and LH levels. It plays a role in increasing ovarian VEGF, and an inhibitory role on granulosa cell apoptosis. As a result, Yi Kun Ning prevents follicle atresia, and hence prevents and retards the aging of ovary.

关 键 词：围绝经期 益坤宁 颗粒细胞凋亡 VEGF 

分 类 号：R-332[医药卫生] R711.75