克罗恩病患者的血清蛋白质组学研究  

Serum proteomic variation study in patients with Crohn disease

作　　者：康亮[1] 杨祖立[2] 刘炜[3] 张泷娟[4] 刘少军[3] 黄美近[1] 黎明涛[3] 汪建平[1] 

机构地区：[1]中山大学附属第六医院(胃肠肛门医院)结直肠外科中山大学胃肠病学研究所,广州510655 [2]中山大学附属第六医院(胃肠肛门医院)胃肠外科,广州510655 [3]中山大学中山医学院蛋白质组学实验室 [4]中山大学附属第一医院外科实验室

出　　处：《中华胃肠外科杂志》2008年第3期266-269,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：广东省自然科学基金（06104601）;广东省自然科学基金博士启动项目（06300805）

摘　　要：目的寻找克罗恩病患者血清中的差异表达蛋白。方法采取克罗恩病患者以及正常成人血清蛋白样本各4例，用不同的CyDye染料交叉标记后依次进行双向胶内差异凝胶电泳（2－D DIGE）、图像分析及基质辅助激光解析离子化飞行时间质谱（MALDI－TOF－MS）鉴定。结果通过比较二者双向电泳图，发现了克罗恩病患者中存在多个表达异常蛋白质点．其中胶号为1058的蛋白质点，在克罗恩病患者血清中比正常成人组表达升高1．68倍（P〈0．05），该蛋白点经质谱鉴定为结合珠蛋白。结论结合珠蛋白在克罗恩病血清的高表达提示在克罗恩病的病程中免疫系统失衡可能起着一定作用。Objective To search differentially expressed proteins in serum of patients with Crohn disease. Methods Serum protein samples from 4 patients with Crohn disease and 8 healthy adults were recruited cross-labeled with variant CyDye, and then followed by two-dimensional differential ingel electrophoresis （2-D DIGE）, image analysis, and identified by matrix-assisted laser desorption/ ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results The 2-D electrophoresis results were compared between the Crohn disease patients and the healthy adults. The spot 1058 expression in serum of Crohn disease patients increased by 1.68 folds as compared with healthy adults （P〈0.05）. The protein was identified as haptoglobin by mass spectrometry. Conclusion Up-regulating expression of haptoglobin in serum of Crohn disease patients may play a role in disequilibrium of immunity system.

关 键 词：克罗恩病 双向胶内差异凝胶电泳 结合珠蛋白 

分 类 号：R574[医药卫生—消化系统]